Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03010 and BPHYT_RS03015 are separated by 34 nucleotides BPHYT_RS03015 and BPHYT_RS03020 are separated by 14 nucleotides BPHYT_RS03020 and BPHYT_RS03025 are separated by 299 nucleotides
BPHYT_RS03010: BPHYT_RS03010 - GntR family transcriptional regulator, at 670,994 to 671,752
_RS03010
BPHYT_RS03015: BPHYT_RS03015 - aldolase, at 671,787 to 672,599
_RS03015
BPHYT_RS03020: BPHYT_RS03020 - hypothetical protein, at 672,614 to 673,414
_RS03020
BPHYT_RS03025: BPHYT_RS03025 - methyl-accepting chemotaxis protein, at 673,714 to 675,363
_RS03025
Position (kb) 672
673
674 Strain fitness (log2 ratio) -2
-1
0
1
2 at 671.714 kb on + strand at 671.714 kb on + strand at 671.715 kb on - strand at 671.715 kb on - strand at 671.802 kb on + strand at 671.803 kb on - strand at 672.291 kb on + strand, within BPHYT_RS03015 at 672.602 kb on + strand at 672.602 kb on + strand at 672.705 kb on + strand, within BPHYT_RS03020 at 672.705 kb on + strand, within BPHYT_RS03020 at 672.705 kb on + strand, within BPHYT_RS03020 at 672.706 kb on - strand, within BPHYT_RS03020 at 672.706 kb on - strand, within BPHYT_RS03020 at 672.894 kb on + strand, within BPHYT_RS03020 at 673.031 kb on + strand, within BPHYT_RS03020 at 673.032 kb on - strand, within BPHYT_RS03020 at 673.185 kb on + strand, within BPHYT_RS03020 at 673.403 kb on + strand at 673.427 kb on + strand at 673.512 kb on + strand at 673.523 kb on - strand at 673.533 kb on - strand at 673.560 kb on - strand at 673.589 kb on + strand at 673.590 kb on - strand at 673.590 kb on - strand at 673.591 kb on + strand at 673.592 kb on - strand at 673.592 kb on - strand at 673.674 kb on - strand at 673.843 kb on + strand at 673.844 kb on - strand at 673.844 kb on - strand at 673.924 kb on + strand, within BPHYT_RS03025 at 674.192 kb on - strand, within BPHYT_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 671,714 + -0.7 671,714 + +0.1 671,715 - -0.3 671,715 - -1.3 671,802 + -0.3 671,803 - +0.3 672,291 + BPHYT_RS03015 0.62 +1.4 672,602 + -0.8 672,602 + -1.1 672,705 + BPHYT_RS03020 0.11 -0.0 672,705 + BPHYT_RS03020 0.11 +0.1 672,705 + BPHYT_RS03020 0.11 -0.2 672,706 - BPHYT_RS03020 0.11 -0.3 672,706 - BPHYT_RS03020 0.11 -0.2 672,894 + BPHYT_RS03020 0.35 +0.3 673,031 + BPHYT_RS03020 0.52 +0.7 673,032 - BPHYT_RS03020 0.52 -2.0 673,185 + BPHYT_RS03020 0.71 -0.5 673,403 + +0.1 673,427 + +0.0 673,512 + -1.2 673,523 - -0.5 673,533 - +0.8 673,560 - +0.8 673,589 + +0.3 673,590 - -0.7 673,590 - -0.6 673,591 + +0.3 673,592 - +0.7 673,592 - +0.5 673,674 - +1.8 673,843 + -0.3 673,844 - +0.4 673,844 - -0.1 673,924 + BPHYT_RS03025 0.13 +0.8 674,192 - BPHYT_RS03025 0.29 -0.2
Or see this region's nucleotide sequence