Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03005 and BPHYT_RS03010 are separated by 39 nucleotides BPHYT_RS03010 and BPHYT_RS03015 are separated by 34 nucleotides BPHYT_RS03015 and BPHYT_RS03020 are separated by 14 nucleotides
BPHYT_RS03005: BPHYT_RS03005 - CoA transferase, at 669,764 to 670,954
_RS03005
BPHYT_RS03010: BPHYT_RS03010 - GntR family transcriptional regulator, at 670,994 to 671,752
_RS03010
BPHYT_RS03015: BPHYT_RS03015 - aldolase, at 671,787 to 672,599
_RS03015
BPHYT_RS03020: BPHYT_RS03020 - hypothetical protein, at 672,614 to 673,414
_RS03020
Position (kb) 670
671
672 Strain fitness (log2 ratio) -1
0
1
2 at 670.095 kb on - strand, within BPHYT_RS03005 at 670.095 kb on - strand, within BPHYT_RS03005 at 670.148 kb on + strand, within BPHYT_RS03005 at 670.190 kb on + strand, within BPHYT_RS03005 at 670.191 kb on - strand, within BPHYT_RS03005 at 670.387 kb on + strand, within BPHYT_RS03005 at 670.388 kb on - strand, within BPHYT_RS03005 at 670.398 kb on - strand, within BPHYT_RS03005 at 670.413 kb on - strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.484 kb on + strand, within BPHYT_RS03005 at 670.953 kb on - strand at 671.033 kb on + strand at 671.033 kb on + strand at 671.285 kb on + strand, within BPHYT_RS03010 at 671.287 kb on + strand, within BPHYT_RS03010 at 671.287 kb on + strand, within BPHYT_RS03010 at 671.450 kb on + strand, within BPHYT_RS03010 at 671.450 kb on + strand, within BPHYT_RS03010 at 671.450 kb on + strand, within BPHYT_RS03010 at 671.450 kb on + strand, within BPHYT_RS03010 at 671.451 kb on - strand, within BPHYT_RS03010 at 671.451 kb on - strand, within BPHYT_RS03010 at 671.451 kb on - strand, within BPHYT_RS03010 at 671.451 kb on - strand, within BPHYT_RS03010 at 671.452 kb on + strand, within BPHYT_RS03010 at 671.452 kb on + strand, within BPHYT_RS03010 at 671.510 kb on + strand, within BPHYT_RS03010 at 671.714 kb on + strand at 671.714 kb on + strand at 671.715 kb on - strand at 671.715 kb on - strand at 671.802 kb on + strand at 671.803 kb on - strand at 672.291 kb on + strand, within BPHYT_RS03015 at 672.602 kb on + strand at 672.602 kb on + strand at 672.705 kb on + strand, within BPHYT_RS03020 at 672.705 kb on + strand, within BPHYT_RS03020 at 672.705 kb on + strand, within BPHYT_RS03020 at 672.706 kb on - strand, within BPHYT_RS03020 at 672.706 kb on - strand, within BPHYT_RS03020
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 670,095 - BPHYT_RS03005 0.28 +0.6 670,095 - BPHYT_RS03005 0.28 -0.1 670,148 + BPHYT_RS03005 0.32 -0.2 670,190 + BPHYT_RS03005 0.36 +0.1 670,191 - BPHYT_RS03005 0.36 -0.1 670,387 + BPHYT_RS03005 0.52 -0.1 670,388 - BPHYT_RS03005 0.52 +2.0 670,398 - BPHYT_RS03005 0.53 -0.1 670,413 - BPHYT_RS03005 0.54 +0.8 670,484 + BPHYT_RS03005 0.60 -0.3 670,484 + BPHYT_RS03005 0.60 +0.1 670,484 + BPHYT_RS03005 0.60 +0.0 670,953 - -0.4 671,033 + -0.3 671,033 + -1.0 671,285 + BPHYT_RS03010 0.38 -0.3 671,287 + BPHYT_RS03010 0.39 +0.8 671,287 + BPHYT_RS03010 0.39 -0.4 671,450 + BPHYT_RS03010 0.60 -1.3 671,450 + BPHYT_RS03010 0.60 +0.3 671,450 + BPHYT_RS03010 0.60 -0.6 671,450 + BPHYT_RS03010 0.60 +0.1 671,451 - BPHYT_RS03010 0.60 -1.2 671,451 - BPHYT_RS03010 0.60 +0.0 671,451 - BPHYT_RS03010 0.60 -1.5 671,451 - BPHYT_RS03010 0.60 -0.1 671,452 + BPHYT_RS03010 0.60 +0.2 671,452 + BPHYT_RS03010 0.60 +0.3 671,510 + BPHYT_RS03010 0.68 -0.6 671,714 + -0.7 671,714 + +0.1 671,715 - -0.3 671,715 - -1.3 671,802 + -0.3 671,803 - +0.3 672,291 + BPHYT_RS03015 0.62 +1.4 672,602 + -0.8 672,602 + -1.1 672,705 + BPHYT_RS03020 0.11 -0.0 672,705 + BPHYT_RS03020 0.11 +0.1 672,705 + BPHYT_RS03020 0.11 -0.2 672,706 - BPHYT_RS03020 0.11 -0.3 672,706 - BPHYT_RS03020 0.11 -0.2
Or see this region's nucleotide sequence