Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28395

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28390 and BPHYT_RS28395 are separated by 281 nucleotides BPHYT_RS28390: BPHYT_RS28390 - hypothetical protein, at 1,922,190 to 1,922,459 _RS28390 BPHYT_RS28395: BPHYT_RS28395 - transporter, at 1,922,741 to 1,923,166 _RS28395 Position (kb) 1922 1923 1924Strain fitness (log2 ratio) -2 -1 0 1at 1922.740 kb on + strandat 1923.189 kb on - strandat 1923.319 kb on - strandat 1923.336 kb on + strandat 1924.003 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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1,922,740 + +1.2
1,923,189 - -2.7
1,923,319 - -0.1
1,923,336 + +0.0
1,924,003 + +0.4

Or see this region's nucleotide sequence