Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26375

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26370 and BPHYT_RS26375 are separated by 321 nucleotidesBPHYT_RS26375 and BPHYT_RS26380 are separated by 5 nucleotides BPHYT_RS26370: BPHYT_RS26370 - porin, at 1,488,601 to 1,489,686 _RS26370 BPHYT_RS26375: BPHYT_RS26375 - ATPase AAA, at 1,490,008 to 1,491,237 _RS26375 BPHYT_RS26380: BPHYT_RS26380 - cobalamin biosynthesis protein CobT, at 1,491,243 to 1,492,991 _RS26380 Position (kb) 1490 1491 1492Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1489.112 kb on + strand, within BPHYT_RS26370at 1489.629 kb on - strandat 1489.631 kb on - strandat 1489.631 kb on - strandat 1489.824 kb on - strandat 1489.824 kb on - strandat 1489.824 kb on - strandat 1489.942 kb on + strandat 1489.943 kb on - strandat 1490.050 kb on + strandat 1490.051 kb on - strandat 1490.875 kb on + strand, within BPHYT_RS26375at 1490.876 kb on - strand, within BPHYT_RS26375at 1491.157 kb on + strandat 1491.307 kb on + strandat 1491.310 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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1,489,112 + BPHYT_RS26370 0.47 -0.5
1,489,629 - +1.1
1,489,631 - +0.0
1,489,631 - -2.8
1,489,824 - -0.5
1,489,824 - +2.6
1,489,824 - +0.0
1,489,942 + +1.4
1,489,943 - +0.4
1,490,050 + +0.9
1,490,051 - +2.0
1,490,875 + BPHYT_RS26375 0.70 -1.6
1,490,876 - BPHYT_RS26375 0.71 -2.0
1,491,157 + -0.1
1,491,307 + +0.4
1,491,310 + -0.1

Or see this region's nucleotide sequence