Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23745

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23740 and BPHYT_RS23745 are separated by 277 nucleotidesBPHYT_RS23745 and BPHYT_RS23750 are separated by 202 nucleotides BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792 _RS23740 BPHYT_RS23745: BPHYT_RS23745 - hypothetical protein, at 895,070 to 895,306 _RS23745 BPHYT_RS23750: BPHYT_RS23750 - phosphonate ABC transporter substrate-binding protein, at 895,509 to 896,597 _RS23750 Position (kb) 895 896Strain fitness (log2 ratio) -1 0 1at 894.238 kb on - strand, within BPHYT_RS23740at 894.388 kb on + strand, within BPHYT_RS23740at 894.389 kb on - strand, within BPHYT_RS23740at 894.473 kb on + strand, within BPHYT_RS23740at 894.474 kb on - strand, within BPHYT_RS23740at 894.474 kb on - strand, within BPHYT_RS23740at 894.474 kb on - strand, within BPHYT_RS23740at 894.583 kb on + strand, within BPHYT_RS23740at 894.835 kb on - strandat 894.939 kb on - strandat 894.981 kb on + strandat 895.040 kb on - strandat 895.040 kb on - strandat 895.040 kb on - strandat 895.061 kb on - strandat 895.305 kb on - strandat 895.635 kb on + strand, within BPHYT_RS23750at 895.635 kb on + strand, within BPHYT_RS23750at 895.662 kb on + strand, within BPHYT_RS23750at 895.843 kb on - strand, within BPHYT_RS23750at 895.857 kb on + strand, within BPHYT_RS23750at 895.858 kb on - strand, within BPHYT_RS23750at 895.920 kb on + strand, within BPHYT_RS23750at 895.921 kb on - strand, within BPHYT_RS23750at 896.148 kb on + strand, within BPHYT_RS23750

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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894,238 - BPHYT_RS23740 0.27 -1.1
894,388 + BPHYT_RS23740 0.46 -1.1
894,389 - BPHYT_RS23740 0.47 +0.7
894,473 + BPHYT_RS23740 0.58 -1.0
894,474 - BPHYT_RS23740 0.58 -0.9
894,474 - BPHYT_RS23740 0.58 -1.0
894,474 - BPHYT_RS23740 0.58 -0.9
894,583 + BPHYT_RS23740 0.72 -0.9
894,835 - -1.1
894,939 - -0.4
894,981 + -0.7
895,040 - +0.6
895,040 - +0.2
895,040 - -1.1
895,061 - +0.1
895,305 - -0.5
895,635 + BPHYT_RS23750 0.12 -0.8
895,635 + BPHYT_RS23750 0.12 +0.2
895,662 + BPHYT_RS23750 0.14 -0.0
895,843 - BPHYT_RS23750 0.31 -0.5
895,857 + BPHYT_RS23750 0.32 -1.3
895,858 - BPHYT_RS23750 0.32 -0.4
895,920 + BPHYT_RS23750 0.38 -0.6
895,921 - BPHYT_RS23750 0.38 -0.6
896,148 + BPHYT_RS23750 0.59 +0.2

Or see this region's nucleotide sequence