Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23510

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23500 and BPHYT_RS23505 are separated by 214 nucleotidesBPHYT_RS23505 and BPHYT_RS23510 are separated by 164 nucleotidesBPHYT_RS23510 and BPHYT_RS23515 are separated by 366 nucleotides BPHYT_RS23500: BPHYT_RS23500 - hypothetical protein, at 838,835 to 840,013 _RS23500 BPHYT_RS23505: BPHYT_RS23505 - hypothetical protein, at 840,228 to 840,611 _RS23505 BPHYT_RS23510: BPHYT_RS23510 - AMP-dependent synthetase, at 840,776 to 842,320 _RS23510 BPHYT_RS23515: BPHYT_RS23515 - porin, at 842,687 to 843,859 _RS23515 Position (kb) 840 841 842 843Strain fitness (log2 ratio) -1 0 1at 840.016 kb on - strandat 840.116 kb on + strandat 840.116 kb on + strandat 840.151 kb on - strandat 840.616 kb on + strandat 841.375 kb on - strand, within BPHYT_RS23510at 841.423 kb on - strand, within BPHYT_RS23510at 842.601 kb on - strandat 842.905 kb on - strand, within BPHYT_RS23515at 842.937 kb on + strand, within BPHYT_RS23515at 842.942 kb on - strand, within BPHYT_RS23515

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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840,016 - +0.0
840,116 + +0.4
840,116 + -0.2
840,151 - -0.4
840,616 + +1.4
841,375 - BPHYT_RS23510 0.39 -0.4
841,423 - BPHYT_RS23510 0.42 -0.7
842,601 - -0.9
842,905 - BPHYT_RS23515 0.19 -0.4
842,937 + BPHYT_RS23515 0.21 +1.4
842,942 - BPHYT_RS23515 0.22 -1.7

Or see this region's nucleotide sequence