Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22955

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotidesBPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotides BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822 _RS22950 BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543 _RS22955 BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145 _RS22960 Position (kb) 707 708 709Strain fitness (log2 ratio) -3 -2 -1 0 1at 706.934 kb on + strand, within BPHYT_RS22950at 707.069 kb on - strand, within BPHYT_RS22950at 707.130 kb on + strand, within BPHYT_RS22950at 707.256 kb on - strand, within BPHYT_RS22950at 707.353 kb on + strand, within BPHYT_RS22950at 707.353 kb on + strand, within BPHYT_RS22950at 707.526 kb on + strand, within BPHYT_RS22950at 707.527 kb on - strand, within BPHYT_RS22950at 707.527 kb on - strand, within BPHYT_RS22950at 707.743 kb on - strandat 707.811 kb on - strandat 708.161 kb on - strand, within BPHYT_RS22955at 708.325 kb on + strand, within BPHYT_RS22955at 708.359 kb on - strand, within BPHYT_RS22955at 708.548 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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706,934 + BPHYT_RS22950 0.34 +0.0
707,069 - BPHYT_RS22950 0.44 -2.5
707,130 + BPHYT_RS22950 0.49 -2.0
707,256 - BPHYT_RS22950 0.58 -1.1
707,353 + BPHYT_RS22950 0.65 -0.3
707,353 + BPHYT_RS22950 0.65 +0.4
707,526 + BPHYT_RS22950 0.78 -3.1
707,527 - BPHYT_RS22950 0.78 -0.2
707,527 - BPHYT_RS22950 0.78 +0.4
707,743 - -0.7
707,811 - +0.4
708,161 - BPHYT_RS22955 0.46 -0.4
708,325 + BPHYT_RS22955 0.69 -0.2
708,359 - BPHYT_RS22955 0.74 -1.0
708,548 - -0.5

Or see this region's nucleotide sequence