Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20100

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20090 and BPHYT_RS20095 are separated by 249 nucleotidesBPHYT_RS20095 and BPHYT_RS20100 overlap by 11 nucleotidesBPHYT_RS20100 and BPHYT_RS20105 are separated by 155 nucleotides BPHYT_RS20090: BPHYT_RS20090 - nitrate ABC transporter substrate-binding protein, at 49,893 to 50,912 _RS20090 BPHYT_RS20095: BPHYT_RS20095 - transcriptional regulator, at 51,162 to 51,839 _RS20095 BPHYT_RS20100: BPHYT_RS20100 - sensor histidine kinase, at 51,829 to 53,223 _RS20100 BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419 _RS20105 Position (kb) 51 52 53 54Strain fitness (log2 ratio) -2 -1 0 1 2at 50.985 kb on + strandat 51.545 kb on - strand, within BPHYT_RS20095at 51.891 kb on - strandat 52.046 kb on + strand, within BPHYT_RS20100at 52.078 kb on + strand, within BPHYT_RS20100at 52.078 kb on + strand, within BPHYT_RS20100at 52.079 kb on - strand, within BPHYT_RS20100at 52.079 kb on - strand, within BPHYT_RS20100at 52.683 kb on + strand, within BPHYT_RS20100at 52.930 kb on - strand, within BPHYT_RS20100at 53.211 kb on + strandat 53.895 kb on + strand, within BPHYT_RS20105at 53.896 kb on - strand, within BPHYT_RS20105at 54.035 kb on + strand, within BPHYT_RS20105at 54.036 kb on - strand, within BPHYT_RS20105

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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50,985 + +0.2
51,545 - BPHYT_RS20095 0.56 -1.2
51,891 - -2.4
52,046 + BPHYT_RS20100 0.16 -1.4
52,078 + BPHYT_RS20100 0.18 -0.9
52,078 + BPHYT_RS20100 0.18 +0.2
52,079 - BPHYT_RS20100 0.18 -1.1
52,079 - BPHYT_RS20100 0.18 -0.2
52,683 + BPHYT_RS20100 0.61 -1.5
52,930 - BPHYT_RS20100 0.79 -0.1
53,211 + +0.4
53,895 + BPHYT_RS20105 0.50 -1.5
53,896 - BPHYT_RS20105 0.50 +2.4
54,035 + BPHYT_RS20105 0.63 +0.2
54,036 - BPHYT_RS20105 0.63 -0.1

Or see this region's nucleotide sequence