Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19505 and BPHYT_RS19510 are separated by 315 nucleotides BPHYT_RS19510 and BPHYT_RS19515 are separated by 53 nucleotides BPHYT_RS19515 and BPHYT_RS19520 are separated by 147 nucleotides
BPHYT_RS19505: BPHYT_RS19505 - LysR family transcriptional regulator, at 4,390,216 to 4,391,115
_RS19505
BPHYT_RS19510: BPHYT_RS19510 - adenylate cyclase, at 4,391,431 to 4,391,967
_RS19510
BPHYT_RS19515: BPHYT_RS19515 - AsnC family transcriptional regulator, at 4,392,021 to 4,392,512
_RS19515
BPHYT_RS19520: BPHYT_RS19520 - phenylalanine 4-monooxygenase, at 4,392,660 to 4,393,550
_RS19520
Position (kb) 4392
4393 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4391.037 kb on + strand at 4391.038 kb on - strand at 4391.171 kb on + strand at 4391.172 kb on - strand at 4391.653 kb on + strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.965 kb on + strand at 4391.965 kb on + strand at 4392.105 kb on + strand, within BPHYT_RS19515 at 4392.106 kb on - strand, within BPHYT_RS19515 at 4392.193 kb on + strand, within BPHYT_RS19515 at 4392.194 kb on - strand, within BPHYT_RS19515 at 4392.436 kb on - strand, within BPHYT_RS19515 at 4392.436 kb on - strand, within BPHYT_RS19515 at 4392.610 kb on + strand at 4392.753 kb on + strand, within BPHYT_RS19520 at 4392.789 kb on + strand, within BPHYT_RS19520 at 4393.035 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.110 kb on + strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.111 kb on - strand, within BPHYT_RS19520 at 4393.123 kb on - strand, within BPHYT_RS19520 at 4393.246 kb on - strand, within BPHYT_RS19520 at 4393.285 kb on - strand, within BPHYT_RS19520 at 4393.405 kb on + strand, within BPHYT_RS19520
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 4,391,037 + -0.4 4,391,038 - +0.3 4,391,171 + +0.3 4,391,172 - +0.9 4,391,653 + BPHYT_RS19510 0.41 -3.1 4,391,654 - BPHYT_RS19510 0.42 -0.2 4,391,654 - BPHYT_RS19510 0.42 -0.4 4,391,749 + BPHYT_RS19510 0.59 -1.2 4,391,749 + BPHYT_RS19510 0.59 +0.1 4,391,749 + BPHYT_RS19510 0.59 -0.3 4,391,749 + BPHYT_RS19510 0.59 +0.2 4,391,749 + BPHYT_RS19510 0.59 +0.0 4,391,750 - BPHYT_RS19510 0.59 +2.8 4,391,750 - BPHYT_RS19510 0.59 -0.6 4,391,750 - BPHYT_RS19510 0.59 +0.5 4,391,795 + BPHYT_RS19510 0.68 -0.8 4,391,795 + BPHYT_RS19510 0.68 -0.1 4,391,965 + +0.2 4,391,965 + -0.2 4,392,105 + BPHYT_RS19515 0.17 +0.9 4,392,106 - BPHYT_RS19515 0.17 -0.3 4,392,193 + BPHYT_RS19515 0.35 -0.8 4,392,194 - BPHYT_RS19515 0.35 +0.3 4,392,436 - BPHYT_RS19515 0.84 -0.6 4,392,436 - BPHYT_RS19515 0.84 -0.8 4,392,610 + +0.7 4,392,753 + BPHYT_RS19520 0.10 +0.9 4,392,789 + BPHYT_RS19520 0.14 +1.1 4,393,035 + BPHYT_RS19520 0.42 -0.2 4,393,110 + BPHYT_RS19520 0.51 +0.1 4,393,110 + BPHYT_RS19520 0.51 +0.0 4,393,110 + BPHYT_RS19520 0.51 -2.8 4,393,111 - BPHYT_RS19520 0.51 -3.3 4,393,111 - BPHYT_RS19520 0.51 +0.4 4,393,111 - BPHYT_RS19520 0.51 +1.3 4,393,111 - BPHYT_RS19520 0.51 -0.9 4,393,111 - BPHYT_RS19520 0.51 +1.1 4,393,123 - BPHYT_RS19520 0.52 -0.4 4,393,246 - BPHYT_RS19520 0.66 +1.8 4,393,285 - BPHYT_RS19520 0.70 -0.6 4,393,405 + BPHYT_RS19520 0.84 -0.3
Or see this region's nucleotide sequence