Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13910

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13900 and BPHYT_RS13905 are separated by 103 nucleotidesBPHYT_RS13905 and BPHYT_RS13910 are separated by 64 nucleotidesBPHYT_RS13910 and BPHYT_RS13915 overlap by 4 nucleotides BPHYT_RS13900: BPHYT_RS13900 - ATPase, at 3,173,082 to 3,174,239 _RS13900 BPHYT_RS13905: BPHYT_RS13905 - ABC transporter ATP-binding protein, at 3,174,343 to 3,175,119 _RS13905 BPHYT_RS13910: BPHYT_RS13910 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data), at 3,175,184 to 3,177,229 _RS13910 BPHYT_RS13915: BPHYT_RS13915 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,177,226 to 3,179,199 _RS13915 Position (kb) 3175 3176 3177 3178Strain fitness (log2 ratio) -2 -1 0 1at 3174.211 kb on - strandat 3175.545 kb on + strand, within BPHYT_RS13910at 3175.631 kb on + strand, within BPHYT_RS13910at 3175.631 kb on + strand, within BPHYT_RS13910at 3175.968 kb on + strand, within BPHYT_RS13910at 3176.654 kb on - strand, within BPHYT_RS13910at 3176.847 kb on - strand, within BPHYT_RS13910at 3177.240 kb on - strandat 3177.683 kb on + strand, within BPHYT_RS13915at 3178.024 kb on + strand, within BPHYT_RS13915

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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3,174,211 - +1.0
3,175,545 + BPHYT_RS13910 0.18 -1.6
3,175,631 + BPHYT_RS13910 0.22 -0.1
3,175,631 + BPHYT_RS13910 0.22 -1.2
3,175,968 + BPHYT_RS13910 0.38 -1.0
3,176,654 - BPHYT_RS13910 0.72 -2.0
3,176,847 - BPHYT_RS13910 0.81 -0.1
3,177,240 - +0.4
3,177,683 + BPHYT_RS13915 0.23 -0.4
3,178,024 + BPHYT_RS13915 0.40 +0.9

Or see this region's nucleotide sequence