Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07485

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07480 and BPHYT_RS07485 are separated by 113 nucleotidesBPHYT_RS07485 and BPHYT_RS07490 are separated by 213 nucleotides BPHYT_RS07480: BPHYT_RS07480 - FAD-dependent oxidoreductase, at 1,700,503 to 1,701,894 _RS07480 BPHYT_RS07485: BPHYT_RS07485 - cupin, at 1,702,008 to 1,702,379 _RS07485 BPHYT_RS07490: BPHYT_RS07490 - aldehyde dehydrogenase, at 1,702,593 to 1,704,029 _RS07490 Position (kb) 1702 1703Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1701.497 kb on - strand, within BPHYT_RS07480at 1702.018 kb on + strandat 1702.019 kb on - strandat 1702.253 kb on - strand, within BPHYT_RS07485at 1702.576 kb on + strandat 1702.578 kb on + strandat 1702.614 kb on + strandat 1702.614 kb on + strandat 1702.615 kb on - strandat 1702.831 kb on - strand, within BPHYT_RS07490at 1703.326 kb on + strand, within BPHYT_RS07490

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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1,701,497 - BPHYT_RS07480 0.71 -1.4
1,702,018 + -0.2
1,702,019 - +0.6
1,702,253 - BPHYT_RS07485 0.66 -0.4
1,702,576 + -0.6
1,702,578 + +0.7
1,702,614 + -0.9
1,702,614 + +1.3
1,702,615 - +2.3
1,702,831 - BPHYT_RS07490 0.17 -4.0
1,703,326 + BPHYT_RS07490 0.51 +0.8

Or see this region's nucleotide sequence