Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06780

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06770 and BPHYT_RS06775 are separated by 142 nucleotidesBPHYT_RS06775 and BPHYT_RS06780 are separated by 57 nucleotidesBPHYT_RS06780 and BPHYT_RS06785 are separated by 33 nucleotides BPHYT_RS06770: BPHYT_RS06770 - aminotransferase, at 1,531,786 to 1,532,958 _RS06770 BPHYT_RS06775: BPHYT_RS06775 - nucleotide-binding protein, at 1,533,101 to 1,533,598 _RS06775 BPHYT_RS06780: BPHYT_RS06780 - hypothetical protein, at 1,533,656 to 1,534,438 _RS06780 BPHYT_RS06785: BPHYT_RS06785 - hypothetical protein, at 1,534,472 to 1,535,626 _RS06785 Position (kb) 1533 1534 1535Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1532.674 kb on - strand, within BPHYT_RS06770at 1532.681 kb on + strand, within BPHYT_RS06770at 1532.693 kb on + strand, within BPHYT_RS06770at 1532.989 kb on - strandat 1533.027 kb on + strandat 1533.027 kb on + strandat 1533.111 kb on - strandat 1533.429 kb on + strand, within BPHYT_RS06775at 1533.429 kb on + strand, within BPHYT_RS06775at 1533.429 kb on + strand, within BPHYT_RS06775at 1533.429 kb on + strand, within BPHYT_RS06775at 1533.430 kb on - strand, within BPHYT_RS06775at 1533.434 kb on + strand, within BPHYT_RS06775at 1533.603 kb on + strandat 1533.656 kb on - strandat 1533.661 kb on - strandat 1533.692 kb on + strandat 1533.693 kb on - strandat 1533.917 kb on + strand, within BPHYT_RS06780at 1533.918 kb on - strand, within BPHYT_RS06780at 1533.963 kb on - strand, within BPHYT_RS06780at 1533.998 kb on + strand, within BPHYT_RS06780at 1534.085 kb on + strand, within BPHYT_RS06780at 1534.086 kb on - strand, within BPHYT_RS06780at 1534.313 kb on + strand, within BPHYT_RS06780at 1534.409 kb on + strandat 1534.409 kb on + strandat 1534.410 kb on - strandat 1534.410 kb on - strandat 1534.413 kb on + strandat 1534.436 kb on + strandat 1534.436 kb on + strandat 1534.486 kb on + strandat 1534.487 kb on - strandat 1534.487 kb on - strandat 1534.704 kb on - strand, within BPHYT_RS06785at 1534.732 kb on + strand, within BPHYT_RS06785at 1534.733 kb on - strand, within BPHYT_RS06785at 1534.733 kb on - strand, within BPHYT_RS06785at 1534.733 kb on - strand, within BPHYT_RS06785at 1534.739 kb on + strand, within BPHYT_RS06785at 1534.786 kb on + strand, within BPHYT_RS06785at 1534.787 kb on - strand, within BPHYT_RS06785at 1534.787 kb on - strand, within BPHYT_RS06785at 1534.790 kb on + strand, within BPHYT_RS06785at 1534.793 kb on + strand, within BPHYT_RS06785at 1534.799 kb on + strand, within BPHYT_RS06785at 1534.811 kb on + strand, within BPHYT_RS06785at 1535.081 kb on + strand, within BPHYT_RS06785at 1535.294 kb on + strand, within BPHYT_RS06785at 1535.294 kb on + strand, within BPHYT_RS06785at 1535.295 kb on - strand, within BPHYT_RS06785at 1535.295 kb on - strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.408 kb on + strand, within BPHYT_RS06785at 1535.409 kb on - strand, within BPHYT_RS06785at 1535.409 kb on - strand, within BPHYT_RS06785at 1535.409 kb on - strand, within BPHYT_RS06785at 1535.409 kb on - strand, within BPHYT_RS06785at 1535.415 kb on - strand, within BPHYT_RS06785at 1535.429 kb on + strand, within BPHYT_RS06785at 1535.430 kb on - strand, within BPHYT_RS06785

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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1,532,674 - BPHYT_RS06770 0.76 -0.5
1,532,681 + BPHYT_RS06770 0.76 +0.3
1,532,693 + BPHYT_RS06770 0.77 +0.4
1,532,989 - -0.1
1,533,027 + +1.3
1,533,027 + +0.7
1,533,111 - -0.3
1,533,429 + BPHYT_RS06775 0.66 +0.7
1,533,429 + BPHYT_RS06775 0.66 -0.8
1,533,429 + BPHYT_RS06775 0.66 -1.4
1,533,429 + BPHYT_RS06775 0.66 -2.2
1,533,430 - BPHYT_RS06775 0.66 -0.7
1,533,434 + BPHYT_RS06775 0.67 +1.0
1,533,603 + +0.0
1,533,656 - +0.3
1,533,661 - -0.3
1,533,692 + -1.3
1,533,693 - +1.9
1,533,917 + BPHYT_RS06780 0.33 +0.3
1,533,918 - BPHYT_RS06780 0.33 -0.1
1,533,963 - BPHYT_RS06780 0.39 -2.9
1,533,998 + BPHYT_RS06780 0.44 +0.3
1,534,085 + BPHYT_RS06780 0.55 +0.4
1,534,086 - BPHYT_RS06780 0.55 +0.7
1,534,313 + BPHYT_RS06780 0.84 +0.3
1,534,409 + -0.1
1,534,409 + +0.3
1,534,410 - +0.3
1,534,410 - +0.8
1,534,413 + -1.0
1,534,436 + -2.1
1,534,436 + +1.2
1,534,486 + +0.2
1,534,487 - +0.1
1,534,487 - -1.7
1,534,704 - BPHYT_RS06785 0.20 -0.6
1,534,732 + BPHYT_RS06785 0.23 -1.2
1,534,733 - BPHYT_RS06785 0.23 -0.2
1,534,733 - BPHYT_RS06785 0.23 -2.0
1,534,733 - BPHYT_RS06785 0.23 +0.5
1,534,739 + BPHYT_RS06785 0.23 -1.0
1,534,786 + BPHYT_RS06785 0.27 -1.4
1,534,787 - BPHYT_RS06785 0.27 +1.4
1,534,787 - BPHYT_RS06785 0.27 -0.3
1,534,790 + BPHYT_RS06785 0.28 +0.4
1,534,793 + BPHYT_RS06785 0.28 +0.1
1,534,799 + BPHYT_RS06785 0.28 -1.7
1,534,811 + BPHYT_RS06785 0.29 +0.8
1,535,081 + BPHYT_RS06785 0.53 -1.0
1,535,294 + BPHYT_RS06785 0.71 +0.2
1,535,294 + BPHYT_RS06785 0.71 +0.4
1,535,295 - BPHYT_RS06785 0.71 +0.7
1,535,295 - BPHYT_RS06785 0.71 -3.3
1,535,408 + BPHYT_RS06785 0.81 +1.0
1,535,408 + BPHYT_RS06785 0.81 -0.1
1,535,408 + BPHYT_RS06785 0.81 +0.8
1,535,408 + BPHYT_RS06785 0.81 -0.1
1,535,408 + BPHYT_RS06785 0.81 +0.1
1,535,408 + BPHYT_RS06785 0.81 +0.3
1,535,408 + BPHYT_RS06785 0.81 -0.0
1,535,408 + BPHYT_RS06785 0.81 +0.2
1,535,408 + BPHYT_RS06785 0.81 +0.4
1,535,408 + BPHYT_RS06785 0.81 +0.6
1,535,409 - BPHYT_RS06785 0.81 -3.2
1,535,409 - BPHYT_RS06785 0.81 +2.7
1,535,409 - BPHYT_RS06785 0.81 -0.3
1,535,409 - BPHYT_RS06785 0.81 -1.0
1,535,415 - BPHYT_RS06785 0.82 +0.3
1,535,429 + BPHYT_RS06785 0.83 +0.7
1,535,430 - BPHYT_RS06785 0.83 +1.2

Or see this region's nucleotide sequence