Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04885 and BPHYT_RS04890 are separated by 17 nucleotides BPHYT_RS04890 and BPHYT_RS04895 overlap by 1 nucleotides BPHYT_RS04895 and BPHYT_RS04900 are separated by 293 nucleotides
BPHYT_RS04885: BPHYT_RS04885 - threonine-phosphate decarboxylase, at 1,123,905 to 1,124,954
_RS04885
BPHYT_RS04890: BPHYT_RS04890 - cobalamin biosynthesis protein CobD, at 1,124,972 to 1,125,907
_RS04890
BPHYT_RS04895: BPHYT_RS04895 - adenosylcobinamide kinase, at 1,125,907 to 1,126,461
_RS04895
BPHYT_RS04900: BPHYT_RS04900 - cobyric acid synthase, at 1,126,755 to 1,128,215
_RS04900
Position (kb) 1125
1126
1127 Strain fitness (log2 ratio) -2
-1
0
1 at 1125.038 kb on - strand at 1125.268 kb on - strand, within BPHYT_RS04890 at 1125.268 kb on - strand, within BPHYT_RS04890 at 1126.017 kb on - strand, within BPHYT_RS04895 at 1126.252 kb on + strand, within BPHYT_RS04895 at 1126.361 kb on + strand, within BPHYT_RS04895 at 1126.361 kb on + strand, within BPHYT_RS04895 at 1126.361 kb on + strand, within BPHYT_RS04895 at 1126.436 kb on - strand at 1126.473 kb on - strand at 1126.732 kb on + strand at 1126.738 kb on + strand at 1126.738 kb on + strand at 1126.784 kb on + strand at 1126.785 kb on - strand at 1126.785 kb on - strand at 1126.941 kb on - strand, within BPHYT_RS04900 at 1127.049 kb on + strand, within BPHYT_RS04900 at 1127.058 kb on + strand, within BPHYT_RS04900 at 1127.058 kb on + strand, within BPHYT_RS04900 at 1127.058 kb on + strand, within BPHYT_RS04900 at 1127.094 kb on + strand, within BPHYT_RS04900 at 1127.094 kb on + strand, within BPHYT_RS04900 at 1127.115 kb on + strand, within BPHYT_RS04900
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 1,125,038 - -1.7 1,125,268 - BPHYT_RS04890 0.32 -1.3 1,125,268 - BPHYT_RS04890 0.32 -2.7 1,126,017 - BPHYT_RS04895 0.20 +0.9 1,126,252 + BPHYT_RS04895 0.62 +0.0 1,126,361 + BPHYT_RS04895 0.82 +0.4 1,126,361 + BPHYT_RS04895 0.82 -1.9 1,126,361 + BPHYT_RS04895 0.82 -0.1 1,126,436 - -0.1 1,126,473 - +0.7 1,126,732 + -0.5 1,126,738 + -0.2 1,126,738 + -0.0 1,126,784 + +0.6 1,126,785 - -0.1 1,126,785 - -0.2 1,126,941 - BPHYT_RS04900 0.13 -0.3 1,127,049 + BPHYT_RS04900 0.20 -0.9 1,127,058 + BPHYT_RS04900 0.21 -1.1 1,127,058 + BPHYT_RS04900 0.21 +1.0 1,127,058 + BPHYT_RS04900 0.21 -0.9 1,127,094 + BPHYT_RS04900 0.23 +0.4 1,127,094 + BPHYT_RS04900 0.23 +0.5 1,127,115 + BPHYT_RS04900 0.25 +1.2
Or see this region's nucleotide sequence