Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03710 and BPHYT_RS03715 are separated by 155 nucleotides BPHYT_RS03715 and BPHYT_RS03720 are separated by 70 nucleotides BPHYT_RS03720 and BPHYT_RS03725 are separated by 516 nucleotides
BPHYT_RS03710: BPHYT_RS03710 - protein tolQ, at 842,623 to 843,300
_RS03710
BPHYT_RS03715: BPHYT_RS03715 - 4-hydroxybenzoyl-CoA thioesterase, at 843,456 to 843,935
_RS03715
BPHYT_RS03720: BPHYT_RS03720 - malonic semialdehyde reductase, at 844,006 to 844,752
_RS03720
BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516
_RS03725
Position (kb) 844
845 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 843.426 kb on - strand at 843.675 kb on + strand, within BPHYT_RS03715 at 843.675 kb on + strand, within BPHYT_RS03715 at 843.675 kb on + strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.676 kb on - strand, within BPHYT_RS03715 at 843.829 kb on + strand, within BPHYT_RS03715 at 843.862 kb on + strand, within BPHYT_RS03715 at 843.865 kb on + strand, within BPHYT_RS03715 at 843.865 kb on + strand, within BPHYT_RS03715 at 843.866 kb on - strand, within BPHYT_RS03715 at 843.887 kb on - strand, within BPHYT_RS03715 at 843.920 kb on + strand at 843.921 kb on - strand at 843.921 kb on - strand at 843.921 kb on - strand at 844.103 kb on + strand, within BPHYT_RS03720 at 844.103 kb on + strand, within BPHYT_RS03720 at 844.104 kb on - strand, within BPHYT_RS03720 at 844.104 kb on - strand, within BPHYT_RS03720 at 844.415 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.903 kb on + strand at 844.903 kb on + strand at 844.903 kb on + strand at 845.015 kb on + strand at 845.207 kb on + strand at 845.214 kb on + strand at 845.268 kb on + strand at 845.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 843,426 - -0.8 843,675 + BPHYT_RS03715 0.46 +0.3 843,675 + BPHYT_RS03715 0.46 -4.0 843,675 + BPHYT_RS03715 0.46 -2.5 843,676 - BPHYT_RS03715 0.46 -0.1 843,676 - BPHYT_RS03715 0.46 -1.1 843,676 - BPHYT_RS03715 0.46 -1.6 843,676 - BPHYT_RS03715 0.46 -0.6 843,829 + BPHYT_RS03715 0.78 -0.9 843,862 + BPHYT_RS03715 0.85 +0.6 843,865 + BPHYT_RS03715 0.85 -0.7 843,865 + BPHYT_RS03715 0.85 -1.5 843,866 - BPHYT_RS03715 0.85 -0.4 843,887 - BPHYT_RS03715 0.90 -1.4 843,920 + +0.4 843,921 - +0.4 843,921 - -1.4 843,921 - +0.3 844,103 + BPHYT_RS03720 0.13 +0.3 844,103 + BPHYT_RS03720 0.13 +0.1 844,104 - BPHYT_RS03720 0.13 +0.2 844,104 - BPHYT_RS03720 0.13 +1.1 844,415 - BPHYT_RS03720 0.55 -1.7 844,602 - BPHYT_RS03720 0.80 +0.4 844,602 - BPHYT_RS03720 0.80 -1.5 844,903 + -0.8 844,903 + -0.7 844,903 + +2.6 845,015 + -0.2 845,207 + -0.5 845,214 + +0.6 845,268 + -1.0 845,269 - -1.7
Or see this region's nucleotide sequence