Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03035 and BPHYT_RS03040 overlap by 14 nucleotides BPHYT_RS03040 and BPHYT_RS03045 are separated by 200 nucleotides BPHYT_RS03045 and BPHYT_RS03050 overlap by 4 nucleotides
BPHYT_RS03035: BPHYT_RS03035 - cytochrome C oxidase subunit I, at 676,241 to 677,866
_RS03035
BPHYT_RS03040: BPHYT_RS03040 - cytochrome C oxidase subunit II, at 677,853 to 678,425
_RS03040
BPHYT_RS03045: BPHYT_RS03045 - alcohol dehydrogenase, at 678,626 to 680,011
_RS03045
BPHYT_RS03050: BPHYT_RS03050 - cytochrome C, at 680,008 to 680,721
_RS03050
Position (kb) 678
679
680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 677.692 kb on - strand, within BPHYT_RS03035 at 677.692 kb on - strand, within BPHYT_RS03035 at 677.692 kb on - strand, within BPHYT_RS03035 at 678.465 kb on + strand at 678.466 kb on - strand at 678.604 kb on - strand at 678.604 kb on - strand at 678.708 kb on + strand at 678.708 kb on + strand at 678.708 kb on + strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.709 kb on - strand at 678.768 kb on + strand, within BPHYT_RS03045 at 678.967 kb on + strand, within BPHYT_RS03045 at 678.967 kb on + strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.968 kb on - strand, within BPHYT_RS03045 at 678.971 kb on - strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.062 kb on + strand, within BPHYT_RS03045 at 679.063 kb on - strand, within BPHYT_RS03045 at 679.077 kb on + strand, within BPHYT_RS03045 at 679.077 kb on + strand, within BPHYT_RS03045 at 679.078 kb on - strand, within BPHYT_RS03045 at 679.101 kb on + strand, within BPHYT_RS03045 at 679.270 kb on + strand, within BPHYT_RS03045 at 679.271 kb on - strand, within BPHYT_RS03045 at 679.320 kb on + strand, within BPHYT_RS03045 at 679.384 kb on - strand, within BPHYT_RS03045 at 679.401 kb on + strand, within BPHYT_RS03045 at 679.402 kb on - strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.524 kb on + strand, within BPHYT_RS03045 at 679.525 kb on - strand, within BPHYT_RS03045 at 679.525 kb on - strand, within BPHYT_RS03045 at 679.656 kb on + strand, within BPHYT_RS03045 at 679.810 kb on + strand, within BPHYT_RS03045 at 679.810 kb on + strand, within BPHYT_RS03045 at 679.811 kb on - strand, within BPHYT_RS03045 at 680.216 kb on + strand, within BPHYT_RS03050 at 680.216 kb on + strand, within BPHYT_RS03050 at 680.432 kb on + strand, within BPHYT_RS03050 at 680.459 kb on + strand, within BPHYT_RS03050 at 680.460 kb on - strand, within BPHYT_RS03050 at 680.475 kb on - strand, within BPHYT_RS03050 at 680.519 kb on + strand, within BPHYT_RS03050 at 680.519 kb on + strand, within BPHYT_RS03050 at 680.552 kb on + strand, within BPHYT_RS03050 at 680.552 kb on + strand, within BPHYT_RS03050 at 680.553 kb on - strand, within BPHYT_RS03050 at 680.671 kb on + strand at 680.671 kb on + strand at 680.678 kb on + strand at 680.679 kb on - strand at 680.706 kb on + strand at 680.706 kb on + strand at 680.747 kb on + strand at 680.748 kb on - strand at 680.760 kb on - strand at 680.810 kb on + strand at 680.810 kb on + strand at 680.810 kb on + strand at 680.839 kb on + strand at 680.841 kb on + strand at 680.842 kb on - strand at 680.921 kb on + strand at 680.921 kb on + strand at 680.922 kb on - strand at 680.923 kb on + strand at 681.007 kb on + strand at 681.007 kb on + strand at 681.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 677,692 - BPHYT_RS03035 0.89 +1.6 677,692 - BPHYT_RS03035 0.89 +0.2 677,692 - BPHYT_RS03035 0.89 +0.4 678,465 + -1.7 678,466 - -0.3 678,604 - +0.7 678,604 - +0.4 678,708 + +0.7 678,708 + +0.0 678,708 + -0.1 678,709 - -0.9 678,709 - -1.0 678,709 - -1.3 678,709 - -1.7 678,709 - +1.6 678,709 - +0.1 678,768 + BPHYT_RS03045 0.10 -0.3 678,967 + BPHYT_RS03045 0.25 -3.1 678,967 + BPHYT_RS03045 0.25 +1.3 678,968 - BPHYT_RS03045 0.25 -0.2 678,968 - BPHYT_RS03045 0.25 +0.0 678,968 - BPHYT_RS03045 0.25 +0.4 678,971 - BPHYT_RS03045 0.25 -0.3 679,062 + BPHYT_RS03045 0.31 +0.3 679,062 + BPHYT_RS03045 0.31 -0.7 679,062 + BPHYT_RS03045 0.31 -1.9 679,063 - BPHYT_RS03045 0.32 +0.0 679,077 + BPHYT_RS03045 0.33 -1.8 679,077 + BPHYT_RS03045 0.33 -0.1 679,078 - BPHYT_RS03045 0.33 -0.2 679,101 + BPHYT_RS03045 0.34 +0.6 679,270 + BPHYT_RS03045 0.46 -1.3 679,271 - BPHYT_RS03045 0.47 -0.7 679,320 + BPHYT_RS03045 0.50 +0.4 679,384 - BPHYT_RS03045 0.55 -0.6 679,401 + BPHYT_RS03045 0.56 -0.5 679,402 - BPHYT_RS03045 0.56 -0.4 679,524 + BPHYT_RS03045 0.65 +0.3 679,524 + BPHYT_RS03045 0.65 -0.5 679,524 + BPHYT_RS03045 0.65 +0.2 679,525 - BPHYT_RS03045 0.65 -0.5 679,525 - BPHYT_RS03045 0.65 +0.1 679,656 + BPHYT_RS03045 0.74 +0.6 679,810 + BPHYT_RS03045 0.85 -0.1 679,810 + BPHYT_RS03045 0.85 +0.2 679,811 - BPHYT_RS03045 0.85 +0.1 680,216 + BPHYT_RS03050 0.29 +0.1 680,216 + BPHYT_RS03050 0.29 +0.1 680,432 + BPHYT_RS03050 0.59 +0.4 680,459 + BPHYT_RS03050 0.63 -2.9 680,460 - BPHYT_RS03050 0.63 +0.2 680,475 - BPHYT_RS03050 0.65 -0.2 680,519 + BPHYT_RS03050 0.72 +0.5 680,519 + BPHYT_RS03050 0.72 +0.1 680,552 + BPHYT_RS03050 0.76 +1.3 680,552 + BPHYT_RS03050 0.76 +0.3 680,553 - BPHYT_RS03050 0.76 +1.3 680,671 + -0.6 680,671 + -1.2 680,678 + -0.1 680,679 - -1.5 680,706 + -1.5 680,706 + +0.4 680,747 + -2.7 680,748 - -0.3 680,760 - +0.0 680,810 + -3.5 680,810 + -1.2 680,810 + -0.6 680,839 + +1.0 680,841 + -1.3 680,842 - +0.1 680,921 + +1.0 680,921 + -1.3 680,922 - -0.3 680,923 + -1.3 681,007 + +0.4 681,007 + -0.1 681,008 - -0.4
Or see this region's nucleotide sequence