Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02005 and BPHYT_RS02010 are separated by 44 nucleotides BPHYT_RS02010 and BPHYT_RS02015 are separated by 95 nucleotides BPHYT_RS02015 and BPHYT_RS02020 are separated by 303 nucleotides
BPHYT_RS02005: BPHYT_RS02005 - diguanylate phosphodiesterase, at 454,840 to 456,108
_RS02005
BPHYT_RS02010: BPHYT_RS02010 - copper homeostasis protein CutC, at 456,153 to 456,848
_RS02010
BPHYT_RS02015: BPHYT_RS02015 - phenylpyruvate ferredoxin oxidoreductase (EC 1.2.7.8) <small>(from data)</small>, at 456,944 to 460,531
_RS02015
BPHYT_RS02020: BPHYT_RS02020 - filamentous hemagglutinin family outer membrane protein, at 460,835 to 463,273
_RS02020
Position (kb) 456
457
458
459
460
461 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 455.950 kb on + strand, within BPHYT_RS02005 at 456.153 kb on - strand at 456.355 kb on - strand, within BPHYT_RS02010 at 456.355 kb on - strand, within BPHYT_RS02010 at 456.370 kb on - strand, within BPHYT_RS02010 at 456.854 kb on + strand at 456.859 kb on - strand at 456.859 kb on - strand at 456.859 kb on - strand at 457.454 kb on + strand, within BPHYT_RS02015 at 457.466 kb on - strand, within BPHYT_RS02015 at 457.614 kb on + strand, within BPHYT_RS02015 at 457.614 kb on + strand, within BPHYT_RS02015 at 457.614 kb on + strand, within BPHYT_RS02015 at 457.615 kb on - strand, within BPHYT_RS02015 at 457.615 kb on - strand, within BPHYT_RS02015 at 457.629 kb on + strand, within BPHYT_RS02015 at 457.629 kb on + strand, within BPHYT_RS02015 at 457.630 kb on - strand at 458.113 kb on + strand, within BPHYT_RS02015 at 458.341 kb on - strand, within BPHYT_RS02015 at 458.490 kb on + strand, within BPHYT_RS02015 at 458.491 kb on - strand, within BPHYT_RS02015 at 458.935 kb on - strand, within BPHYT_RS02015 at 458.947 kb on - strand, within BPHYT_RS02015 at 459.006 kb on + strand, within BPHYT_RS02015 at 459.190 kb on + strand, within BPHYT_RS02015 at 459.191 kb on - strand, within BPHYT_RS02015 at 459.191 kb on - strand, within BPHYT_RS02015 at 459.191 kb on - strand, within BPHYT_RS02015 at 459.441 kb on + strand, within BPHYT_RS02015 at 459.442 kb on - strand, within BPHYT_RS02015 at 459.442 kb on - strand, within BPHYT_RS02015 at 459.447 kb on + strand, within BPHYT_RS02015 at 459.447 kb on + strand, within BPHYT_RS02015 at 459.447 kb on + strand, within BPHYT_RS02015 at 459.447 kb on + strand, within BPHYT_RS02015 at 459.448 kb on - strand, within BPHYT_RS02015 at 459.448 kb on - strand, within BPHYT_RS02015 at 459.448 kb on - strand, within BPHYT_RS02015 at 459.568 kb on - strand, within BPHYT_RS02015 at 459.568 kb on - strand, within BPHYT_RS02015 at 459.907 kb on - strand, within BPHYT_RS02015 at 459.948 kb on + strand, within BPHYT_RS02015 at 460.485 kb on + strand at 460.486 kb on - strand at 460.549 kb on - strand at 460.823 kb on + strand at 460.836 kb on + strand at 460.837 kb on - strand at 460.861 kb on - strand at 460.867 kb on - strand at 460.881 kb on + strand at 460.894 kb on - strand at 460.900 kb on + strand at 460.901 kb on - strand at 461.186 kb on + strand, within BPHYT_RS02020 at 461.187 kb on - strand, within BPHYT_RS02020 at 461.246 kb on - strand, within BPHYT_RS02020 at 461.310 kb on - strand, within BPHYT_RS02020
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 455,950 + BPHYT_RS02005 0.87 -0.0 456,153 - -1.8 456,355 - BPHYT_RS02010 0.29 -0.4 456,355 - BPHYT_RS02010 0.29 +1.7 456,370 - BPHYT_RS02010 0.31 -3.9 456,854 + -2.0 456,859 - -2.1 456,859 - -0.3 456,859 - -1.4 457,454 + BPHYT_RS02015 0.14 -2.2 457,466 - BPHYT_RS02015 0.15 +2.0 457,614 + BPHYT_RS02015 0.19 -0.5 457,614 + BPHYT_RS02015 0.19 -0.2 457,614 + BPHYT_RS02015 0.19 -0.7 457,615 - BPHYT_RS02015 0.19 -0.4 457,615 - BPHYT_RS02015 0.19 -2.0 457,629 + BPHYT_RS02015 0.19 -1.9 457,629 + BPHYT_RS02015 0.19 -0.8 457,630 - +0.3 458,113 + BPHYT_RS02015 0.33 -0.9 458,341 - BPHYT_RS02015 0.39 -0.4 458,490 + BPHYT_RS02015 0.43 -0.5 458,491 - BPHYT_RS02015 0.43 -0.7 458,935 - BPHYT_RS02015 0.55 -0.3 458,947 - BPHYT_RS02015 0.56 -0.8 459,006 + BPHYT_RS02015 0.57 -0.2 459,190 + BPHYT_RS02015 0.63 -1.3 459,191 - BPHYT_RS02015 0.63 -0.4 459,191 - BPHYT_RS02015 0.63 -0.2 459,191 - BPHYT_RS02015 0.63 -0.6 459,441 + BPHYT_RS02015 0.70 -0.1 459,442 - BPHYT_RS02015 0.70 -1.5 459,442 - BPHYT_RS02015 0.70 -0.7 459,447 + BPHYT_RS02015 0.70 -0.6 459,447 + BPHYT_RS02015 0.70 -0.9 459,447 + BPHYT_RS02015 0.70 -3.8 459,447 + BPHYT_RS02015 0.70 +0.4 459,448 - BPHYT_RS02015 0.70 -0.2 459,448 - BPHYT_RS02015 0.70 -1.0 459,448 - BPHYT_RS02015 0.70 -0.2 459,568 - BPHYT_RS02015 0.73 -0.5 459,568 - BPHYT_RS02015 0.73 -1.4 459,907 - BPHYT_RS02015 0.83 +0.2 459,948 + BPHYT_RS02015 0.84 -0.4 460,485 + +0.4 460,486 - -1.1 460,549 - -1.1 460,823 + -0.3 460,836 + +0.2 460,837 - -1.4 460,861 - -0.4 460,867 - -0.7 460,881 + +0.1 460,894 - -0.6 460,900 + +1.1 460,901 - -2.2 461,186 + BPHYT_RS02020 0.14 -0.9 461,187 - BPHYT_RS02020 0.14 -0.8 461,246 - BPHYT_RS02020 0.17 -0.3 461,310 - BPHYT_RS02020 0.19 +0.1
Or see this region's nucleotide sequence