Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01620 and BPHYT_RS01625 overlap by 4 nucleotides BPHYT_RS01625 and BPHYT_RS01630 are separated by 143 nucleotides BPHYT_RS01630 and BPHYT_RS01635 are separated by 9 nucleotides
BPHYT_RS01620: BPHYT_RS01620 - sensor histidine kinase, at 368,130 to 369,440
_RS01620
BPHYT_RS01625: BPHYT_RS01625 - chemotaxis protein CheY, at 369,437 to 369,979
_RS01625
BPHYT_RS01630: BPHYT_RS01630 - ATP-dependent protease ATPase subunit HslU, at 370,123 to 371,469
_RS01630
BPHYT_RS01635: BPHYT_RS01635 - ATP-dependent protease subunit HslV, at 371,479 to 372,015
_RS01635
Position (kb) 370
371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 369.172 kb on + strand, within BPHYT_RS01620 at 369.172 kb on + strand, within BPHYT_RS01620 at 369.173 kb on - strand, within BPHYT_RS01620 at 369.173 kb on - strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.239 kb on + strand, within BPHYT_RS01620 at 369.240 kb on - strand, within BPHYT_RS01620 at 369.240 kb on - strand, within BPHYT_RS01620 at 369.267 kb on + strand, within BPHYT_RS01620 at 369.359 kb on + strand at 369.737 kb on + strand, within BPHYT_RS01625 at 369.737 kb on + strand, within BPHYT_RS01625 at 369.865 kb on + strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.866 kb on - strand, within BPHYT_RS01625 at 369.977 kb on + strand at 369.977 kb on + strand at 369.978 kb on - strand at 370.016 kb on - strand at 370.268 kb on + strand, within BPHYT_RS01630 at 370.392 kb on - strand, within BPHYT_RS01630 at 371.199 kb on - strand, within BPHYT_RS01630 at 371.695 kb on - strand, within BPHYT_RS01635 at 372.016 kb on - strand at 372.016 kb on - strand at 372.064 kb on - strand at 372.147 kb on + strand at 372.170 kb on - strand at 372.191 kb on + strand at 372.192 kb on - strand at 372.295 kb on + strand at 372.296 kb on - strand at 372.304 kb on + strand at 372.305 kb on - strand at 372.371 kb on + strand at 372.371 kb on + strand at 372.372 kb on - strand at 372.399 kb on - strand at 372.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 369,172 + BPHYT_RS01620 0.79 -1.7 369,172 + BPHYT_RS01620 0.79 -0.0 369,173 - BPHYT_RS01620 0.80 -3.1 369,173 - BPHYT_RS01620 0.80 -1.7 369,239 + BPHYT_RS01620 0.85 -2.0 369,239 + BPHYT_RS01620 0.85 -0.8 369,239 + BPHYT_RS01620 0.85 -0.5 369,240 - BPHYT_RS01620 0.85 +0.2 369,240 - BPHYT_RS01620 0.85 -0.1 369,267 + BPHYT_RS01620 0.87 -3.5 369,359 + -1.8 369,737 + BPHYT_RS01625 0.55 -2.2 369,737 + BPHYT_RS01625 0.55 -3.2 369,865 + BPHYT_RS01625 0.79 -1.4 369,866 - BPHYT_RS01625 0.79 -2.1 369,866 - BPHYT_RS01625 0.79 -2.6 369,866 - BPHYT_RS01625 0.79 +0.6 369,977 + -0.1 369,977 + +0.2 369,978 - +0.5 370,016 - +0.2 370,268 + BPHYT_RS01630 0.11 +2.5 370,392 - BPHYT_RS01630 0.20 +0.2 371,199 - BPHYT_RS01630 0.80 -2.3 371,695 - BPHYT_RS01635 0.40 +1.4 372,016 - -0.8 372,016 - +0.7 372,064 - +0.3 372,147 + -0.2 372,170 - +0.1 372,191 + -0.2 372,192 - +0.9 372,295 + -0.3 372,296 - +0.2 372,304 + -0.4 372,305 - +0.1 372,371 + -1.6 372,371 + +0.1 372,372 - -0.3 372,399 - -1.1 372,399 - +0.7
Or see this region's nucleotide sequence