Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS01205

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS01200 and BPHYT_RS01205 are separated by 21 nucleotidesBPHYT_RS01205 and BPHYT_RS01210 are separated by 32 nucleotides BPHYT_RS01200: BPHYT_RS01200 - hypothetical protein, at 277,162 to 279,012 _RS01200 BPHYT_RS01205: BPHYT_RS01205 - hypothetical protein, at 279,034 to 279,222 _RS01205 BPHYT_RS01210: BPHYT_RS01210 - hypothetical protein, at 279,255 to 280,337 _RS01210 Position (kb) 279 280Strain fitness (log2 ratio) -3 -2 -1 0 1at 278.037 kb on - strand, within BPHYT_RS01200at 278.080 kb on + strand, within BPHYT_RS01200at 278.281 kb on + strand, within BPHYT_RS01200at 278.282 kb on - strand, within BPHYT_RS01200at 278.296 kb on + strand, within BPHYT_RS01200at 278.345 kb on - strand, within BPHYT_RS01200at 278.377 kb on + strand, within BPHYT_RS01200at 278.378 kb on - strand, within BPHYT_RS01200at 278.378 kb on - strand, within BPHYT_RS01200at 278.378 kb on - strand, within BPHYT_RS01200at 278.608 kb on + strand, within BPHYT_RS01200at 278.609 kb on - strand, within BPHYT_RS01200at 279.034 kb on - strandat 279.169 kb on + strand, within BPHYT_RS01205at 279.362 kb on + strandat 279.363 kb on - strandat 279.387 kb on + strand, within BPHYT_RS01210at 279.407 kb on + strand, within BPHYT_RS01210at 279.687 kb on - strand, within BPHYT_RS01210at 279.687 kb on - strand, within BPHYT_RS01210at 280.080 kb on - strand, within BPHYT_RS01210

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Per-strain Table

Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
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278,037 - BPHYT_RS01200 0.47 +0.4
278,080 + BPHYT_RS01200 0.50 -0.8
278,281 + BPHYT_RS01200 0.60 -0.5
278,282 - BPHYT_RS01200 0.61 -0.1
278,296 + BPHYT_RS01200 0.61 +0.3
278,345 - BPHYT_RS01200 0.64 -1.5
278,377 + BPHYT_RS01200 0.66 -0.2
278,378 - BPHYT_RS01200 0.66 -0.6
278,378 - BPHYT_RS01200 0.66 +0.0
278,378 - BPHYT_RS01200 0.66 -0.1
278,608 + BPHYT_RS01200 0.78 -0.3
278,609 - BPHYT_RS01200 0.78 -2.9
279,034 - +0.0
279,169 + BPHYT_RS01205 0.71 -0.9
279,362 + -1.2
279,363 - +0.8
279,387 + BPHYT_RS01210 0.12 -0.3
279,407 + BPHYT_RS01210 0.14 -2.4
279,687 - BPHYT_RS01210 0.40 +0.8
279,687 - BPHYT_RS01210 0.40 -1.5
280,080 - BPHYT_RS01210 0.76 -0.3

Or see this region's nucleotide sequence