Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01195 and BPHYT_RS01200 are separated by 52 nucleotides BPHYT_RS01200 and BPHYT_RS01205 are separated by 21 nucleotides BPHYT_RS01205 and BPHYT_RS01210 are separated by 32 nucleotides
BPHYT_RS01195: BPHYT_RS01195 - hypothetical protein, at 275,139 to 277,109
_RS01195
BPHYT_RS01200: BPHYT_RS01200 - hypothetical protein, at 277,162 to 279,012
_RS01200
BPHYT_RS01205: BPHYT_RS01205 - hypothetical protein, at 279,034 to 279,222
_RS01205
BPHYT_RS01210: BPHYT_RS01210 - hypothetical protein, at 279,255 to 280,337
_RS01210
Position (kb) 277
278
279
280 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 276.211 kb on - strand, within BPHYT_RS01195 at 276.440 kb on + strand, within BPHYT_RS01195 at 276.567 kb on + strand, within BPHYT_RS01195 at 276.576 kb on + strand, within BPHYT_RS01195 at 276.577 kb on - strand, within BPHYT_RS01195 at 276.577 kb on - strand, within BPHYT_RS01195 at 276.856 kb on - strand, within BPHYT_RS01195 at 277.009 kb on + strand at 277.009 kb on + strand at 277.071 kb on - strand at 277.090 kb on + strand at 277.135 kb on + strand at 277.136 kb on - strand at 277.150 kb on - strand at 277.186 kb on - strand at 277.195 kb on + strand at 277.199 kb on - strand at 277.199 kb on - strand at 277.266 kb on + strand at 277.267 kb on - strand at 277.366 kb on + strand, within BPHYT_RS01200 at 277.408 kb on + strand, within BPHYT_RS01200 at 277.408 kb on + strand, within BPHYT_RS01200 at 277.409 kb on - strand, within BPHYT_RS01200 at 277.594 kb on + strand, within BPHYT_RS01200 at 277.751 kb on - strand, within BPHYT_RS01200 at 277.751 kb on - strand, within BPHYT_RS01200 at 277.754 kb on - strand, within BPHYT_RS01200 at 277.808 kb on - strand, within BPHYT_RS01200 at 277.808 kb on - strand, within BPHYT_RS01200 at 277.892 kb on - strand, within BPHYT_RS01200 at 277.892 kb on - strand, within BPHYT_RS01200 at 278.037 kb on - strand, within BPHYT_RS01200 at 278.080 kb on + strand, within BPHYT_RS01200 at 278.281 kb on + strand, within BPHYT_RS01200 at 278.282 kb on - strand, within BPHYT_RS01200 at 278.296 kb on + strand, within BPHYT_RS01200 at 278.345 kb on - strand, within BPHYT_RS01200 at 278.377 kb on + strand, within BPHYT_RS01200 at 278.378 kb on - strand, within BPHYT_RS01200 at 278.378 kb on - strand, within BPHYT_RS01200 at 278.378 kb on - strand, within BPHYT_RS01200 at 278.608 kb on + strand, within BPHYT_RS01200 at 278.609 kb on - strand, within BPHYT_RS01200 at 279.034 kb on - strand at 279.169 kb on + strand, within BPHYT_RS01205 at 279.362 kb on + strand at 279.363 kb on - strand at 279.387 kb on + strand, within BPHYT_RS01210 at 279.407 kb on + strand, within BPHYT_RS01210 at 279.687 kb on - strand, within BPHYT_RS01210 at 279.687 kb on - strand, within BPHYT_RS01210
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 276,211 - BPHYT_RS01195 0.54 -0.1 276,440 + BPHYT_RS01195 0.66 +0.4 276,567 + BPHYT_RS01195 0.72 +0.8 276,576 + BPHYT_RS01195 0.73 -1.0 276,577 - BPHYT_RS01195 0.73 -0.1 276,577 - BPHYT_RS01195 0.73 +0.6 276,856 - BPHYT_RS01195 0.87 -0.4 277,009 + -0.3 277,009 + +0.3 277,071 - -0.4 277,090 + -0.1 277,135 + -1.1 277,136 - -2.1 277,150 - +0.0 277,186 - -1.5 277,195 + -0.8 277,199 - -0.3 277,199 - -0.4 277,266 + -0.2 277,267 - -1.6 277,366 + BPHYT_RS01200 0.11 +0.3 277,408 + BPHYT_RS01200 0.13 -0.1 277,408 + BPHYT_RS01200 0.13 +0.1 277,409 - BPHYT_RS01200 0.13 +2.2 277,594 + BPHYT_RS01200 0.23 -0.0 277,751 - BPHYT_RS01200 0.32 -0.7 277,751 - BPHYT_RS01200 0.32 -2.4 277,754 - BPHYT_RS01200 0.32 -1.4 277,808 - BPHYT_RS01200 0.35 -0.4 277,808 - BPHYT_RS01200 0.35 +0.0 277,892 - BPHYT_RS01200 0.39 -0.5 277,892 - BPHYT_RS01200 0.39 -0.2 278,037 - BPHYT_RS01200 0.47 +0.4 278,080 + BPHYT_RS01200 0.50 -0.8 278,281 + BPHYT_RS01200 0.60 -0.5 278,282 - BPHYT_RS01200 0.61 -0.1 278,296 + BPHYT_RS01200 0.61 +0.3 278,345 - BPHYT_RS01200 0.64 -1.5 278,377 + BPHYT_RS01200 0.66 -0.2 278,378 - BPHYT_RS01200 0.66 -0.6 278,378 - BPHYT_RS01200 0.66 +0.0 278,378 - BPHYT_RS01200 0.66 -0.1 278,608 + BPHYT_RS01200 0.78 -0.3 278,609 - BPHYT_RS01200 0.78 -2.9 279,034 - +0.0 279,169 + BPHYT_RS01205 0.71 -0.9 279,362 + -1.2 279,363 - +0.8 279,387 + BPHYT_RS01210 0.12 -0.3 279,407 + BPHYT_RS01210 0.14 -2.4 279,687 - BPHYT_RS01210 0.40 +0.8 279,687 - BPHYT_RS01210 0.40 -1.5
Or see this region's nucleotide sequence