Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01185 and BPHYT_RS01190 are separated by 153 nucleotides BPHYT_RS01190 and BPHYT_RS01195 are separated by 800 nucleotides
BPHYT_RS01185: BPHYT_RS01185 - sodium:sulfate symporter, at 271,984 to 273,444
_RS01185
BPHYT_RS01190: BPHYT_RS01190 - hypothetical protein, at 273,598 to 274,338
_RS01190
BPHYT_RS01195: BPHYT_RS01195 - hypothetical protein, at 275,139 to 277,109
_RS01195
Position (kb) 273
274
275 Strain fitness (log2 ratio) -1
0
1
2 at 272.599 kb on + strand, within BPHYT_RS01185 at 272.599 kb on + strand, within BPHYT_RS01185 at 272.599 kb on + strand, within BPHYT_RS01185 at 272.599 kb on + strand at 272.599 kb on + strand, within BPHYT_RS01185 at 272.600 kb on - strand, within BPHYT_RS01185 at 272.600 kb on - strand, within BPHYT_RS01185 at 272.600 kb on - strand, within BPHYT_RS01185 at 272.600 kb on - strand, within BPHYT_RS01185 at 272.601 kb on + strand, within BPHYT_RS01185 at 272.671 kb on + strand, within BPHYT_RS01185 at 272.689 kb on + strand, within BPHYT_RS01185 at 272.698 kb on + strand, within BPHYT_RS01185 at 272.698 kb on + strand, within BPHYT_RS01185 at 272.699 kb on - strand, within BPHYT_RS01185 at 273.010 kb on + strand, within BPHYT_RS01185 at 273.083 kb on + strand, within BPHYT_RS01185 at 273.328 kb on - strand at 273.443 kb on - strand at 273.597 kb on + strand at 273.597 kb on + strand at 273.598 kb on - strand at 273.598 kb on - strand at 273.598 kb on - strand at 274.376 kb on - strand at 274.573 kb on + strand at 274.659 kb on - strand at 274.671 kb on - strand at 274.672 kb on + strand at 274.702 kb on - strand at 274.835 kb on + strand at 274.836 kb on - strand at 274.887 kb on - strand at 274.943 kb on + strand at 275.272 kb on - strand at 275.276 kb on + strand at 275.277 kb on - strand at 275.277 kb on - strand at 275.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 272,599 + BPHYT_RS01185 0.42 +0.0 272,599 + BPHYT_RS01185 0.42 -0.5 272,599 + BPHYT_RS01185 0.42 -0.2 272,599 + +0.4 272,599 + BPHYT_RS01185 0.42 -0.4 272,600 - BPHYT_RS01185 0.42 -0.7 272,600 - BPHYT_RS01185 0.42 +0.7 272,600 - BPHYT_RS01185 0.42 -1.5 272,600 - BPHYT_RS01185 0.42 -0.6 272,601 + BPHYT_RS01185 0.42 -0.5 272,671 + BPHYT_RS01185 0.47 -0.5 272,689 + BPHYT_RS01185 0.48 +1.2 272,698 + BPHYT_RS01185 0.49 +0.8 272,698 + BPHYT_RS01185 0.49 -0.1 272,699 - BPHYT_RS01185 0.49 +0.2 273,010 + BPHYT_RS01185 0.70 +0.2 273,083 + BPHYT_RS01185 0.75 -0.9 273,328 - -0.7 273,443 - -1.7 273,597 + +0.4 273,597 + -0.4 273,598 - -0.1 273,598 - +0.8 273,598 - +0.9 274,376 - +0.6 274,573 + -1.6 274,659 - +0.1 274,671 - +0.3 274,672 + +0.6 274,702 - -1.6 274,835 + +0.4 274,836 - +1.8 274,887 - -1.3 274,943 + -0.1 275,272 - +1.1 275,276 + +0.5 275,277 - -0.1 275,277 - +0.2 275,304 + -0.3
Or see this region's nucleotide sequence