Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01000 and BPHYT_RS01005 overlap by 4 nucleotides BPHYT_RS01005 and BPHYT_RS01010 are separated by 263 nucleotides BPHYT_RS01010 and BPHYT_RS01015 are separated by 37 nucleotides
BPHYT_RS01000: BPHYT_RS01000 - integrase, at 228,694 to 231,300
_RS01000
BPHYT_RS01005: BPHYT_RS01005 - alpha/beta hydrolase, at 231,297 to 231,959
_RS01005
BPHYT_RS01010: BPHYT_RS01010 - hypothetical protein, at 232,223 to 232,504
_RS01010
BPHYT_RS01015: BPHYT_RS01015 - hypothetical protein, at 232,542 to 233,501
_RS01015
Position (kb) 231
232 Strain fitness (log2 ratio) -2
-1
0
1
2 at 230.584 kb on + strand, within BPHYT_RS01000 at 230.682 kb on - strand, within BPHYT_RS01000 at 230.877 kb on - strand, within BPHYT_RS01000 at 231.003 kb on - strand, within BPHYT_RS01000 at 231.377 kb on + strand, within BPHYT_RS01005 at 231.378 kb on - strand, within BPHYT_RS01005 at 231.382 kb on + strand, within BPHYT_RS01005 at 231.499 kb on - strand, within BPHYT_RS01005 at 231.519 kb on + strand, within BPHYT_RS01005 at 231.550 kb on - strand, within BPHYT_RS01005 at 231.600 kb on + strand, within BPHYT_RS01005 at 231.862 kb on + strand, within BPHYT_RS01005 at 231.863 kb on - strand, within BPHYT_RS01005 at 231.992 kb on + strand at 231.993 kb on - strand at 231.993 kb on - strand at 231.998 kb on + strand at 231.998 kb on + strand at 231.999 kb on - strand at 231.999 kb on - strand at 232.064 kb on + strand at 232.064 kb on + strand at 232.064 kb on + strand at 232.064 kb on + strand at 232.064 kb on + strand at 232.065 kb on - strand at 232.065 kb on - strand at 232.065 kb on - strand at 232.065 kb on - strand at 232.134 kb on - strand at 232.149 kb on + strand at 232.149 kb on + strand at 232.206 kb on + strand at 232.306 kb on + strand, within BPHYT_RS01010 at 232.482 kb on + strand at 232.585 kb on + strand at 232.585 kb on + strand at 232.723 kb on + strand, within BPHYT_RS01015 at 232.803 kb on - strand, within BPHYT_RS01015 at 232.857 kb on - strand, within BPHYT_RS01015 at 232.956 kb on + strand, within BPHYT_RS01015
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 230,584 + BPHYT_RS01000 0.72 +2.2 230,682 - BPHYT_RS01000 0.76 +0.0 230,877 - BPHYT_RS01000 0.84 -0.6 231,003 - BPHYT_RS01000 0.89 -1.3 231,377 + BPHYT_RS01005 0.12 -0.5 231,378 - BPHYT_RS01005 0.12 -0.5 231,382 + BPHYT_RS01005 0.13 -0.1 231,499 - BPHYT_RS01005 0.30 -1.6 231,519 + BPHYT_RS01005 0.33 -1.1 231,550 - BPHYT_RS01005 0.38 +0.6 231,600 + BPHYT_RS01005 0.46 -1.5 231,862 + BPHYT_RS01005 0.85 -0.3 231,863 - BPHYT_RS01005 0.85 -0.2 231,992 + -0.5 231,993 - -0.6 231,993 - -0.3 231,998 + +0.0 231,998 + +0.7 231,999 - -0.2 231,999 - -0.5 232,064 + +2.0 232,064 + -0.4 232,064 + -0.3 232,064 + -2.0 232,064 + -0.1 232,065 - +0.4 232,065 - -2.4 232,065 - +0.9 232,065 - +0.2 232,134 - +0.1 232,149 + +0.7 232,149 + -0.4 232,206 + -1.6 232,306 + BPHYT_RS01010 0.29 -0.3 232,482 + -0.1 232,585 + -0.5 232,585 + +0.9 232,723 + BPHYT_RS01015 0.19 -0.2 232,803 - BPHYT_RS01015 0.27 +0.1 232,857 - BPHYT_RS01015 0.33 -0.2 232,956 + BPHYT_RS01015 0.43 +0.3
Or see this region's nucleotide sequence