Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00050 and BPHYT_RS00055 are separated by 72 nucleotides BPHYT_RS00055 and BPHYT_RS00060 are separated by 104 nucleotides BPHYT_RS00060 and BPHYT_RS00065 overlap by 4 nucleotides BPHYT_RS00065 and BPHYT_RS00070 are separated by 251 nucleotides
BPHYT_RS00050: BPHYT_RS00050 - hypothetical protein, at 14,958 to 15,692
_RS00050
BPHYT_RS00055: BPHYT_RS00055 - hypothetical protein, at 15,765 to 16,016
_RS00055
BPHYT_RS00060: BPHYT_RS00060 - hypothetical protein, at 16,121 to 18,958
_RS00060
BPHYT_RS00065: BPHYT_RS00065 - hypothetical protein, at 18,955 to 19,692
_RS00065
BPHYT_RS00070: BPHYT_RS00070 - hypothetical protein, at 19,944 to 21,122
_RS00070
Position (kb) 16
17
18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 15.244 kb on + strand, within BPHYT_RS00050 at 15.289 kb on - strand, within BPHYT_RS00050 at 15.327 kb on + strand, within BPHYT_RS00050 at 15.585 kb on + strand, within BPHYT_RS00050 at 15.585 kb on + strand, within BPHYT_RS00050 at 15.585 kb on + strand, within BPHYT_RS00050 at 15.585 kb on + strand, within BPHYT_RS00050 at 15.664 kb on - strand at 15.673 kb on - strand at 15.723 kb on + strand at 15.723 kb on + strand at 15.724 kb on - strand at 15.794 kb on + strand, within BPHYT_RS00055 at 15.795 kb on - strand, within BPHYT_RS00055 at 16.052 kb on - strand at 16.187 kb on + strand at 16.277 kb on + strand at 16.278 kb on - strand at 16.278 kb on - strand at 16.310 kb on + strand at 16.311 kb on - strand at 16.311 kb on - strand at 16.319 kb on + strand at 16.346 kb on + strand at 16.346 kb on + strand at 16.359 kb on - strand at 16.469 kb on - strand, within BPHYT_RS00060 at 16.524 kb on - strand, within BPHYT_RS00060 at 16.553 kb on + strand, within BPHYT_RS00060 at 16.553 kb on + strand, within BPHYT_RS00060 at 16.679 kb on + strand, within BPHYT_RS00060 at 16.680 kb on - strand, within BPHYT_RS00060 at 16.680 kb on - strand, within BPHYT_RS00060 at 16.795 kb on + strand, within BPHYT_RS00060 at 16.796 kb on - strand, within BPHYT_RS00060 at 16.798 kb on - strand, within BPHYT_RS00060 at 16.798 kb on - strand, within BPHYT_RS00060 at 16.798 kb on - strand, within BPHYT_RS00060 at 16.973 kb on + strand, within BPHYT_RS00060 at 16.973 kb on + strand, within BPHYT_RS00060 at 16.973 kb on + strand, within BPHYT_RS00060 at 16.974 kb on - strand, within BPHYT_RS00060 at 16.974 kb on - strand, within BPHYT_RS00060 at 16.974 kb on - strand, within BPHYT_RS00060 at 16.977 kb on + strand, within BPHYT_RS00060 at 17.060 kb on - strand, within BPHYT_RS00060 at 17.215 kb on + strand, within BPHYT_RS00060 at 17.246 kb on + strand, within BPHYT_RS00060 at 17.247 kb on - strand, within BPHYT_RS00060 at 17.255 kb on - strand, within BPHYT_RS00060 at 17.260 kb on + strand, within BPHYT_RS00060 at 17.302 kb on + strand, within BPHYT_RS00060 at 17.303 kb on - strand, within BPHYT_RS00060 at 17.397 kb on - strand, within BPHYT_RS00060 at 17.795 kb on - strand, within BPHYT_RS00060 at 17.795 kb on - strand, within BPHYT_RS00060 at 17.839 kb on - strand, within BPHYT_RS00060 at 17.865 kb on + strand at 17.865 kb on + strand, within BPHYT_RS00060 at 17.866 kb on - strand, within BPHYT_RS00060 at 17.878 kb on + strand, within BPHYT_RS00060 at 18.018 kb on - strand, within BPHYT_RS00060 at 18.209 kb on + strand, within BPHYT_RS00060 at 18.319 kb on - strand, within BPHYT_RS00060 at 18.431 kb on + strand, within BPHYT_RS00060 at 18.439 kb on + strand, within BPHYT_RS00060 at 18.458 kb on - strand, within BPHYT_RS00060 at 18.489 kb on + strand, within BPHYT_RS00060 at 18.697 kb on - strand at 18.697 kb on - strand at 18.730 kb on + strand at 18.731 kb on - strand at 18.778 kb on - strand at 18.806 kb on + strand at 18.847 kb on + strand at 18.868 kb on + strand at 19.731 kb on - strand at 19.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 remove 15,244 + BPHYT_RS00050 0.39 +0.2 15,289 - BPHYT_RS00050 0.45 +2.2 15,327 + BPHYT_RS00050 0.50 -0.7 15,585 + BPHYT_RS00050 0.85 +0.5 15,585 + BPHYT_RS00050 0.85 -0.2 15,585 + BPHYT_RS00050 0.85 -0.1 15,585 + BPHYT_RS00050 0.85 +0.1 15,664 - -0.6 15,673 - -0.1 15,723 + -0.9 15,723 + -1.4 15,724 - -1.5 15,794 + BPHYT_RS00055 0.12 -3.4 15,795 - BPHYT_RS00055 0.12 -0.8 16,052 - +0.2 16,187 + +0.1 16,277 + -0.9 16,278 - -1.2 16,278 - -2.4 16,310 + -0.5 16,311 - -0.9 16,311 - -0.3 16,319 + -1.4 16,346 + -0.1 16,346 + +2.2 16,359 - +1.1 16,469 - BPHYT_RS00060 0.12 +0.2 16,524 - BPHYT_RS00060 0.14 -1.4 16,553 + BPHYT_RS00060 0.15 -0.7 16,553 + BPHYT_RS00060 0.15 +0.0 16,679 + BPHYT_RS00060 0.20 -0.3 16,680 - BPHYT_RS00060 0.20 -1.2 16,680 - BPHYT_RS00060 0.20 -0.1 16,795 + BPHYT_RS00060 0.24 -0.6 16,796 - BPHYT_RS00060 0.24 -0.8 16,798 - BPHYT_RS00060 0.24 +0.0 16,798 - BPHYT_RS00060 0.24 +0.3 16,798 - BPHYT_RS00060 0.24 -0.2 16,973 + BPHYT_RS00060 0.30 +0.9 16,973 + BPHYT_RS00060 0.30 -0.2 16,973 + BPHYT_RS00060 0.30 +0.2 16,974 - BPHYT_RS00060 0.30 -0.3 16,974 - BPHYT_RS00060 0.30 -0.9 16,974 - BPHYT_RS00060 0.30 +1.1 16,977 + BPHYT_RS00060 0.30 +0.1 17,060 - BPHYT_RS00060 0.33 -0.0 17,215 + BPHYT_RS00060 0.39 -0.4 17,246 + BPHYT_RS00060 0.40 -0.4 17,247 - BPHYT_RS00060 0.40 -0.8 17,255 - BPHYT_RS00060 0.40 +2.2 17,260 + BPHYT_RS00060 0.40 +1.0 17,302 + BPHYT_RS00060 0.42 -0.7 17,303 - BPHYT_RS00060 0.42 -1.1 17,397 - BPHYT_RS00060 0.45 +2.0 17,795 - BPHYT_RS00060 0.59 -1.1 17,795 - BPHYT_RS00060 0.59 -0.5 17,839 - BPHYT_RS00060 0.61 -1.6 17,865 + +0.2 17,865 + BPHYT_RS00060 0.61 -0.1 17,866 - BPHYT_RS00060 0.61 +2.2 17,878 + BPHYT_RS00060 0.62 +0.5 18,018 - BPHYT_RS00060 0.67 +0.1 18,209 + BPHYT_RS00060 0.74 -1.0 18,319 - BPHYT_RS00060 0.77 -1.0 18,431 + BPHYT_RS00060 0.81 -0.0 18,439 + BPHYT_RS00060 0.82 -1.4 18,458 - BPHYT_RS00060 0.82 +0.6 18,489 + BPHYT_RS00060 0.83 -1.1 18,697 - +0.1 18,697 - -1.2 18,730 + +0.1 18,731 - +0.4 18,778 - -2.4 18,806 + +0.6 18,847 + -0.4 18,868 + +0.1 19,731 - +0.6 19,731 - +0.4
Or see this region's nucleotide sequence