Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00055

Experiment: Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
14,937	+				-1.7
15,244	+		BPHYT_RS00050	0.39	+0.2
15,289	-		BPHYT_RS00050	0.45	+2.2
15,327	+		BPHYT_RS00050	0.50	-0.7
15,585	+		BPHYT_RS00050	0.85	+0.5
15,585	+		BPHYT_RS00050	0.85	-0.2
15,585	+		BPHYT_RS00050	0.85	-0.1
15,585	+		BPHYT_RS00050	0.85	+0.1
15,664	-				-0.6
15,673	-				-0.1
15,723	+				-0.9
15,723	+				-1.4
15,724	-				-1.5
15,794	+		BPHYT_RS00055	0.12	-3.4
15,795	-		BPHYT_RS00055	0.12	-0.8
16,052	-				+0.2
16,187	+				+0.1
16,277	+				-0.9
16,278	-				-1.2
16,278	-				-2.4
16,310	+				-0.5
16,311	-				-0.9
16,311	-				-0.3
16,319	+				-1.4
16,346	+				-0.1
16,346	+				+2.2
16,359	-				+1.1
16,469	-		BPHYT_RS00060	0.12	+0.2
16,524	-		BPHYT_RS00060	0.14	-1.4
16,553	+		BPHYT_RS00060	0.15	-0.7
16,553	+		BPHYT_RS00060	0.15	+0.0
16,679	+		BPHYT_RS00060	0.20	-0.3
16,680	-		BPHYT_RS00060	0.20	-1.2
16,680	-		BPHYT_RS00060	0.20	-0.1
16,795	+		BPHYT_RS00060	0.24	-0.6
16,796	-		BPHYT_RS00060	0.24	-0.8
16,798	-		BPHYT_RS00060	0.24	+0.0
16,798	-		BPHYT_RS00060	0.24	+0.3
16,798	-		BPHYT_RS00060	0.24	-0.2
16,973	+		BPHYT_RS00060	0.30	+0.9
16,973	+		BPHYT_RS00060	0.30	-0.2
16,973	+		BPHYT_RS00060	0.30	+0.2
16,974	-		BPHYT_RS00060	0.30	-0.3
16,974	-		BPHYT_RS00060	0.30	-0.9
16,974	-		BPHYT_RS00060	0.30	+1.1
16,977	+		BPHYT_RS00060	0.30	+0.1