Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34620 and BPHYT_RS34625 are separated by 43 nucleotides BPHYT_RS34625 and BPHYT_RS34630 are separated by 63 nucleotides BPHYT_RS34630 and BPHYT_RS34635 are separated by 212 nucleotides BPHYT_RS34635 and BPHYT_RS34640 are separated by 13 nucleotides
BPHYT_RS34620: BPHYT_RS34620 - hypothetical protein, at 3,377,318 to 3,377,593
_RS34620
BPHYT_RS34625: BPHYT_RS34625 - hypothetical protein, at 3,377,637 to 3,377,993
_RS34625
BPHYT_RS34630: BPHYT_RS34630 - hypothetical protein, at 3,378,057 to 3,378,575
_RS34630
BPHYT_RS34635: BPHYT_RS34635 - 50S ribosomal protein L21, at 3,378,788 to 3,379,267
_RS34635
BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946
_RS34640
Position (kb)
3378
3379 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3377.074 kb on - strand at 3377.142 kb on + strand at 3377.142 kb on + strand at 3377.193 kb on - strand at 3377.243 kb on - strand at 3377.357 kb on - strand, within BPHYT_RS34620 at 3377.503 kb on + strand, within BPHYT_RS34620 at 3377.592 kb on - strand at 3377.592 kb on - strand at 3377.611 kb on + strand at 3377.611 kb on + strand at 3377.612 kb on - strand at 3377.877 kb on - strand, within BPHYT_RS34625 at 3377.912 kb on + strand, within BPHYT_RS34625 at 3377.929 kb on + strand, within BPHYT_RS34625 at 3377.930 kb on - strand, within BPHYT_RS34625 at 3378.036 kb on - strand at 3378.151 kb on + strand, within BPHYT_RS34630 at 3378.151 kb on + strand, within BPHYT_RS34630 at 3378.220 kb on + strand, within BPHYT_RS34630 at 3378.221 kb on - strand, within BPHYT_RS34630 at 3378.225 kb on - strand, within BPHYT_RS34630 at 3378.225 kb on - strand, within BPHYT_RS34630 at 3378.225 kb on - strand, within BPHYT_RS34630 at 3378.225 kb on - strand, within BPHYT_RS34630 at 3378.226 kb on + strand, within BPHYT_RS34630 at 3378.308 kb on - strand, within BPHYT_RS34630 at 3378.311 kb on - strand, within BPHYT_RS34630 at 3378.439 kb on + strand, within BPHYT_RS34630 at 3378.611 kb on + strand at 3378.611 kb on + strand at 3378.612 kb on - strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.629 kb on - strand at 3378.629 kb on - strand at 3378.629 kb on - strand at 3378.849 kb on + strand, within BPHYT_RS34635 at 3378.984 kb on + strand, within BPHYT_RS34635 at 3378.985 kb on - strand, within BPHYT_RS34635 at 3379.270 kb on + strand at 3379.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 3,377,074 - -0.4 3,377,142 + -1.8 3,377,142 + +0.2 3,377,193 - -1.1 3,377,243 - +1.3 3,377,357 - BPHYT_RS34620 0.14 -0.3 3,377,503 + BPHYT_RS34620 0.67 +1.8 3,377,592 - -0.1 3,377,592 - -0.3 3,377,611 + -2.7 3,377,611 + -1.1 3,377,612 - -0.9 3,377,877 - BPHYT_RS34625 0.67 +0.2 3,377,912 + BPHYT_RS34625 0.77 +1.1 3,377,929 + BPHYT_RS34625 0.82 -1.2 3,377,930 - BPHYT_RS34625 0.82 +0.3 3,378,036 - +0.4 3,378,151 + BPHYT_RS34630 0.18 -0.1 3,378,151 + BPHYT_RS34630 0.18 -0.1 3,378,220 + BPHYT_RS34630 0.31 +0.4 3,378,221 - BPHYT_RS34630 0.32 +0.7 3,378,225 - BPHYT_RS34630 0.32 -1.7 3,378,225 - BPHYT_RS34630 0.32 +1.9 3,378,225 - BPHYT_RS34630 0.32 +1.2 3,378,225 - BPHYT_RS34630 0.32 +0.5 3,378,226 + BPHYT_RS34630 0.33 +1.3 3,378,308 - BPHYT_RS34630 0.48 +0.1 3,378,311 - BPHYT_RS34630 0.49 -0.0 3,378,439 + BPHYT_RS34630 0.74 +0.1 3,378,611 + +0.3 3,378,611 + +0.3 3,378,612 - +1.2 3,378,628 + -0.4 3,378,628 + -0.3 3,378,628 + -0.2 3,378,628 + -0.1 3,378,629 - -3.8 3,378,629 - +1.4 3,378,629 - -0.9 3,378,849 + BPHYT_RS34635 0.13 +0.2 3,378,984 + BPHYT_RS34635 0.41 -0.3 3,378,985 - BPHYT_RS34635 0.41 -2.0 3,379,270 + -0.1 3,379,271 - -0.0
Or see this region's nucleotide sequence