Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32425

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32420 and BPHYT_RS32425 are separated by 35 nucleotidesBPHYT_RS32425 and BPHYT_RS32430 are separated by 111 nucleotidesBPHYT_RS32430 and BPHYT_RS32435 overlap by 14 nucleotides BPHYT_RS32420: BPHYT_RS32420 - selenocysteine synthase, at 2,846,390 to 2,847,820 _RS32420 BPHYT_RS32425: BPHYT_RS32425 - formate dehydrogenase accessory protein FdhE, at 2,847,856 to 2,848,851 _RS32425 BPHYT_RS32430: BPHYT_RS32430 - formate dehydrogenase-N subunit gamma, at 2,848,963 to 2,849,619 _RS32430 BPHYT_RS32435: BPHYT_RS32435 - formate dehydrogenase-N subunit beta, at 2,849,606 to 2,850,538 _RS32435 Position (kb) 2847 2848 2849Strain fitness (log2 ratio) -3 -2 -1 0 1at 2847.114 kb on + strand, within BPHYT_RS32420at 2847.114 kb on + strand, within BPHYT_RS32420at 2847.115 kb on - strand, within BPHYT_RS32420at 2847.171 kb on + strand, within BPHYT_RS32420at 2847.610 kb on + strand, within BPHYT_RS32420at 2847.718 kb on - strandat 2847.809 kb on + strandat 2848.035 kb on - strand, within BPHYT_RS32425at 2848.223 kb on + strand, within BPHYT_RS32425at 2848.223 kb on + strand, within BPHYT_RS32425at 2848.224 kb on - strand, within BPHYT_RS32425at 2848.224 kb on - strand, within BPHYT_RS32425at 2848.352 kb on - strand, within BPHYT_RS32425at 2848.517 kb on - strand, within BPHYT_RS32425at 2848.517 kb on - strand, within BPHYT_RS32425at 2848.854 kb on + strandat 2848.951 kb on - strandat 2848.951 kb on - strandat 2849.018 kb on + strandat 2849.018 kb on + strandat 2849.076 kb on - strand, within BPHYT_RS32430at 2849.266 kb on - strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.470 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.642 kb on + strandat 2849.713 kb on - strand, within BPHYT_RS32435

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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2,847,114 + BPHYT_RS32420 0.51 -0.9
2,847,114 + BPHYT_RS32420 0.51 -0.1
2,847,115 - BPHYT_RS32420 0.51 +1.0
2,847,171 + BPHYT_RS32420 0.55 -1.9
2,847,610 + BPHYT_RS32420 0.85 -0.2
2,847,718 - -0.2
2,847,809 + +0.6
2,848,035 - BPHYT_RS32425 0.18 -0.2
2,848,223 + BPHYT_RS32425 0.37 -0.0
2,848,223 + BPHYT_RS32425 0.37 +1.1
2,848,224 - BPHYT_RS32425 0.37 +1.1
2,848,224 - BPHYT_RS32425 0.37 +0.9
2,848,352 - BPHYT_RS32425 0.50 +0.0
2,848,517 - BPHYT_RS32425 0.66 +0.5
2,848,517 - BPHYT_RS32425 0.66 -0.2
2,848,854 + -0.8
2,848,951 - +0.6
2,848,951 - +0.1
2,849,018 + -0.0
2,849,018 + -1.8
2,849,076 - BPHYT_RS32430 0.17 +0.1
2,849,266 - BPHYT_RS32430 0.46 -0.8
2,849,348 + BPHYT_RS32430 0.59 -1.4
2,849,348 + BPHYT_RS32430 0.59 -2.2
2,849,348 + BPHYT_RS32430 0.59 -0.5
2,849,349 - BPHYT_RS32430 0.59 +0.2
2,849,349 - BPHYT_RS32430 0.59 -0.2
2,849,349 - BPHYT_RS32430 0.59 -3.1
2,849,470 - BPHYT_RS32430 0.77 -1.2
2,849,542 - BPHYT_RS32430 0.88 +0.9
2,849,542 - BPHYT_RS32430 0.88 +0.4
2,849,642 + +0.5
2,849,713 - BPHYT_RS32435 0.11 +1.0

Or see this region's nucleotide sequence