Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32330

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32325 and BPHYT_RS32330 are separated by 73 nucleotidesBPHYT_RS32330 and BPHYT_RS32335 are separated by 333 nucleotidesBPHYT_RS32335 and BPHYT_RS32340 are separated by 133 nucleotides BPHYT_RS32325: BPHYT_RS32325 - 5-oxoprolinase, at 2,823,288 to 2,825,012 _RS32325 BPHYT_RS32330: BPHYT_RS32330 - 5-oxoprolinase, at 2,825,086 to 2,827,122 _RS32330 BPHYT_RS32335: BPHYT_RS32335 - AsnC family transcriptional regulator, at 2,827,456 to 2,827,881 _RS32335 BPHYT_RS32340: BPHYT_RS32340 - LrgA family protein, at 2,828,015 to 2,828,419 _RS32340 Position (kb) 2825 2826 2827 2828Strain fitness (log2 ratio) -3 -2 -1 0 1at 2824.335 kb on - strand, within BPHYT_RS32325at 2824.686 kb on - strand, within BPHYT_RS32325at 2825.295 kb on + strand, within BPHYT_RS32330at 2825.853 kb on + strand, within BPHYT_RS32330at 2825.854 kb on - strand, within BPHYT_RS32330at 2825.883 kb on - strand, within BPHYT_RS32330at 2826.008 kb on + strand, within BPHYT_RS32330at 2826.094 kb on + strand, within BPHYT_RS32330at 2826.467 kb on + strand, within BPHYT_RS32330at 2826.517 kb on - strand, within BPHYT_RS32330at 2826.524 kb on + strand, within BPHYT_RS32330at 2826.618 kb on + strand, within BPHYT_RS32330at 2826.844 kb on + strand, within BPHYT_RS32330at 2826.845 kb on - strand, within BPHYT_RS32330at 2827.105 kb on + strandat 2827.544 kb on - strand, within BPHYT_RS32335at 2827.580 kb on - strand, within BPHYT_RS32335at 2828.037 kb on + strandat 2828.109 kb on + strand, within BPHYT_RS32340at 2828.110 kb on - strand, within BPHYT_RS32340

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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2,824,335 - BPHYT_RS32325 0.61 +0.1
2,824,686 - BPHYT_RS32325 0.81 +1.1
2,825,295 + BPHYT_RS32330 0.10 -0.4
2,825,853 + BPHYT_RS32330 0.38 -2.6
2,825,854 - BPHYT_RS32330 0.38 -3.2
2,825,883 - BPHYT_RS32330 0.39 -0.5
2,826,008 + BPHYT_RS32330 0.45 +1.0
2,826,094 + BPHYT_RS32330 0.49 -0.8
2,826,467 + BPHYT_RS32330 0.68 -3.2
2,826,517 - BPHYT_RS32330 0.70 +1.6
2,826,524 + BPHYT_RS32330 0.71 +0.6
2,826,618 + BPHYT_RS32330 0.75 -1.0
2,826,844 + BPHYT_RS32330 0.86 +0.2
2,826,845 - BPHYT_RS32330 0.86 +0.0
2,827,105 + -1.0
2,827,544 - BPHYT_RS32335 0.21 +0.3
2,827,580 - BPHYT_RS32335 0.29 +1.4
2,828,037 + +0.5
2,828,109 + BPHYT_RS32340 0.23 +0.2
2,828,110 - BPHYT_RS32340 0.23 -0.8

Or see this region's nucleotide sequence