Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28050 and BPHYT_RS28055 overlap by 4 nucleotides BPHYT_RS28055 and BPHYT_RS28060 are separated by 129 nucleotides BPHYT_RS28060 and BPHYT_RS28065 are separated by 288 nucleotides BPHYT_RS28065 and BPHYT_RS28070 are separated by 152 nucleotides
BPHYT_RS28050: BPHYT_RS28050 - serine protease, at 1,857,303 to 1,858,916
_RS28050
BPHYT_RS28055: BPHYT_RS28055 - membrane protein, at 1,858,913 to 1,859,686
_RS28055
BPHYT_RS28060: BPHYT_RS28060 - hypothetical protein, at 1,859,816 to 1,860,073
_RS28060
BPHYT_RS28065: BPHYT_RS28065 - hypothetical protein, at 1,860,362 to 1,860,859
_RS28065
BPHYT_RS28070: BPHYT_RS28070 - adenine permease, at 1,861,012 to 1,862,313
_RS28070
Position (kb) 1859
1860
1861 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1859.184 kb on - strand, within BPHYT_RS28055 at 1859.231 kb on + strand, within BPHYT_RS28055 at 1859.232 kb on - strand, within BPHYT_RS28055 at 1859.653 kb on - strand at 1859.773 kb on + strand at 1859.804 kb on + strand at 1859.866 kb on + strand, within BPHYT_RS28060 at 1859.866 kb on + strand, within BPHYT_RS28060 at 1859.867 kb on - strand, within BPHYT_RS28060 at 1859.900 kb on + strand, within BPHYT_RS28060 at 1859.900 kb on + strand, within BPHYT_RS28060 at 1859.901 kb on - strand, within BPHYT_RS28060 at 1859.901 kb on - strand, within BPHYT_RS28060 at 1860.282 kb on - strand at 1860.319 kb on + strand at 1860.319 kb on + strand at 1860.320 kb on - strand at 1860.416 kb on + strand, within BPHYT_RS28065 at 1860.417 kb on - strand, within BPHYT_RS28065 at 1861.022 kb on + strand at 1861.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 1,859,184 - BPHYT_RS28055 0.35 -0.5 1,859,231 + BPHYT_RS28055 0.41 -0.5 1,859,232 - BPHYT_RS28055 0.41 +0.2 1,859,653 - -0.7 1,859,773 + +0.8 1,859,804 + +0.4 1,859,866 + BPHYT_RS28060 0.19 +0.0 1,859,866 + BPHYT_RS28060 0.19 -0.7 1,859,867 - BPHYT_RS28060 0.20 +0.8 1,859,900 + BPHYT_RS28060 0.33 +0.1 1,859,900 + BPHYT_RS28060 0.33 -0.4 1,859,901 - BPHYT_RS28060 0.33 +0.6 1,859,901 - BPHYT_RS28060 0.33 +0.2 1,860,282 - +0.5 1,860,319 + -0.0 1,860,319 + -0.5 1,860,320 - +0.6 1,860,416 + BPHYT_RS28065 0.11 -1.0 1,860,417 - BPHYT_RS28065 0.11 -3.4 1,861,022 + +0.8 1,861,023 - +1.1
Or see this region's nucleotide sequence