Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27310

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27305 and BPHYT_RS27310 are separated by 445 nucleotidesBPHYT_RS27310 and BPHYT_RS27315 are separated by 67 nucleotides BPHYT_RS27305: BPHYT_RS27305 - hypothetical protein, at 1,702,148 to 1,702,495 _RS27305 BPHYT_RS27310: BPHYT_RS27310 - LysR family transcriptional regulator, at 1,702,941 to 1,703,912 _RS27310 BPHYT_RS27315: BPHYT_RS27315 - DSBA oxidoreductase, at 1,703,980 to 1,704,633 _RS27315 Position (kb) 1702 1703 1704Strain fitness (log2 ratio) -3 -2 -1 0 1at 1702.030 kb on - strandat 1702.050 kb on - strandat 1702.274 kb on + strand, within BPHYT_RS27305at 1702.424 kb on + strand, within BPHYT_RS27305at 1702.715 kb on - strandat 1702.720 kb on - strandat 1702.865 kb on - strandat 1702.943 kb on - strandat 1703.838 kb on - strandat 1703.838 kb on - strandat 1704.101 kb on - strand, within BPHYT_RS27315at 1704.359 kb on - strand, within BPHYT_RS27315at 1704.359 kb on - strand, within BPHYT_RS27315at 1704.464 kb on + strand, within BPHYT_RS27315at 1704.864 kb on + strandat 1704.864 kb on + strandat 1704.864 kb on + strandat 1704.864 kb on + strandat 1704.865 kb on - strandat 1704.900 kb on + strandat 1704.901 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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1,702,030 - -0.6
1,702,050 - -0.2
1,702,274 + BPHYT_RS27305 0.36 -0.2
1,702,424 + BPHYT_RS27305 0.79 -1.1
1,702,715 - -3.5
1,702,720 - -0.6
1,702,865 - +0.8
1,702,943 - +0.1
1,703,838 - -1.1
1,703,838 - +0.2
1,704,101 - BPHYT_RS27315 0.19 +0.4
1,704,359 - BPHYT_RS27315 0.58 +0.0
1,704,359 - BPHYT_RS27315 0.58 +0.5
1,704,464 + BPHYT_RS27315 0.74 -0.2
1,704,864 + -0.2
1,704,864 + -0.7
1,704,864 + +0.5
1,704,864 + +0.2
1,704,865 - +1.2
1,704,900 + -1.5
1,704,901 - +0.7

Or see this region's nucleotide sequence