Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27295

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27290 and BPHYT_RS27295 are separated by 949 nucleotidesBPHYT_RS27295 and BPHYT_RS27300 are separated by 247 nucleotides BPHYT_RS27290: BPHYT_RS27290 - pyridine nucleotide-disulfide oxidoreductase, at 1,697,995 to 1,699,257 _RS27290 BPHYT_RS27295: BPHYT_RS27295 - hypothetical protein, at 1,700,207 to 1,700,773 _RS27295 BPHYT_RS27300: BPHYT_RS27300 - hypothetical protein, at 1,701,021 to 1,701,809 _RS27300 Position (kb) 1700 1701Strain fitness (log2 ratio) -2 -1 0 1 2at 1699.217 kb on + strandat 1699.340 kb on - strandat 1699.937 kb on - strandat 1700.013 kb on + strandat 1700.043 kb on - strandat 1700.065 kb on + strandat 1700.420 kb on + strand, within BPHYT_RS27295at 1700.438 kb on - strand, within BPHYT_RS27295at 1700.949 kb on + strandat 1700.949 kb on + strandat 1700.949 kb on + strandat 1700.955 kb on + strandat 1700.955 kb on + strandat 1700.956 kb on - strandat 1701.023 kb on - strandat 1701.080 kb on + strandat 1701.222 kb on + strand, within BPHYT_RS27300at 1701.245 kb on + strand, within BPHYT_RS27300at 1701.511 kb on + strand, within BPHYT_RS27300at 1701.512 kb on - strand, within BPHYT_RS27300

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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1,699,217 + -1.9
1,699,340 - -0.9
1,699,937 - +1.4
1,700,013 + +0.3
1,700,043 - -0.3
1,700,065 + +0.6
1,700,420 + BPHYT_RS27295 0.38 -1.1
1,700,438 - BPHYT_RS27295 0.41 -1.7
1,700,949 + +0.4
1,700,949 + -0.1
1,700,949 + +0.3
1,700,955 + +1.6
1,700,955 + -0.0
1,700,956 - +0.3
1,701,023 - +0.3
1,701,080 + -2.3
1,701,222 + BPHYT_RS27300 0.25 +1.0
1,701,245 + BPHYT_RS27300 0.28 +0.1
1,701,511 + BPHYT_RS27300 0.62 +1.8
1,701,512 - BPHYT_RS27300 0.62 -1.1

Or see this region's nucleotide sequence