Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26175 and BPHYT_RS26180 are separated by 20 nucleotides BPHYT_RS26180 and BPHYT_RS26185 are separated by 11 nucleotides BPHYT_RS26185 and BPHYT_RS26190 are separated by 11 nucleotides BPHYT_RS26190 and BPHYT_RS26195 are separated by 152 nucleotides BPHYT_RS26195 and BPHYT_RS26200 are separated by 3 nucleotides
BPHYT_RS26175: BPHYT_RS26175 - XRE family transcriptional regulator, at 1,446,377 to 1,446,949
_RS26175
BPHYT_RS26180: BPHYT_RS26180 - acetyltransferase, at 1,446,970 to 1,447,506
_RS26180
BPHYT_RS26185: BPHYT_RS26185 - thioesterase, at 1,447,518 to 1,447,913
_RS26185
BPHYT_RS26190: BPHYT_RS26190 - lysine transporter LysE, at 1,447,925 to 1,448,557
_RS26190
BPHYT_RS26195: BPHYT_RS26195 - transporter, at 1,448,710 to 1,448,970
_RS26195
BPHYT_RS26200: BPHYT_RS26200 - acetate kinase, at 1,448,974 to 1,450,134
_RS26200
Position (kb)
1447
1448
1449 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1447.492 kb on + strand at 1447.493 kb on - strand at 1447.502 kb on + strand at 1447.503 kb on - strand at 1447.503 kb on - strand at 1447.672 kb on + strand, within BPHYT_RS26185 at 1448.166 kb on + strand, within BPHYT_RS26190 at 1448.167 kb on - strand, within BPHYT_RS26190 at 1448.301 kb on + strand, within BPHYT_RS26190 at 1448.478 kb on + strand, within BPHYT_RS26190 at 1448.478 kb on + strand, within BPHYT_RS26190 at 1448.600 kb on - strand at 1448.686 kb on - strand at 1449.053 kb on - strand at 1449.381 kb on - strand, within BPHYT_RS26200
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 1,447,492 + +0.7 1,447,493 - +0.6 1,447,502 + -3.5 1,447,503 - +0.8 1,447,503 - -3.1 1,447,672 + BPHYT_RS26185 0.39 -1.1 1,448,166 + BPHYT_RS26190 0.38 -0.6 1,448,167 - BPHYT_RS26190 0.38 -1.3 1,448,301 + BPHYT_RS26190 0.59 -0.2 1,448,478 + BPHYT_RS26190 0.87 -0.5 1,448,478 + BPHYT_RS26190 0.87 -0.7 1,448,600 - -0.3 1,448,686 - +0.2 1,449,053 - +1.5 1,449,381 - BPHYT_RS26200 0.35 +0.4
Or see this region's nucleotide sequence