Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24440

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24435 and BPHYT_RS24440 are separated by 4 nucleotidesBPHYT_RS24440 and BPHYT_RS24445 overlap by 4 nucleotides BPHYT_RS24435: BPHYT_RS24435 - cell envelope biogenesis protein OmpA, at 1,048,325 to 1,049,287 _RS24435 BPHYT_RS24440: BPHYT_RS24440 - type VI secretion protein, at 1,049,292 to 1,050,284 _RS24440 BPHYT_RS24445: BPHYT_RS24445 - type VI secretion protein VasK, at 1,050,281 to 1,054,147 _RS24445 Position (kb) 1049 1050 1051Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1048.458 kb on + strand, within BPHYT_RS24435at 1048.459 kb on - strand, within BPHYT_RS24435at 1048.499 kb on - strand, within BPHYT_RS24435at 1048.691 kb on + strand, within BPHYT_RS24435at 1048.691 kb on + strand, within BPHYT_RS24435at 1048.691 kb on + strand, within BPHYT_RS24435at 1048.692 kb on - strand, within BPHYT_RS24435at 1048.697 kb on + strand, within BPHYT_RS24435at 1048.716 kb on + strand, within BPHYT_RS24435at 1048.774 kb on + strand, within BPHYT_RS24435at 1048.938 kb on + strand, within BPHYT_RS24435at 1049.450 kb on - strand, within BPHYT_RS24440at 1049.807 kb on + strand, within BPHYT_RS24440at 1050.054 kb on - strand, within BPHYT_RS24440at 1050.054 kb on - strand, within BPHYT_RS24440at 1051.105 kb on - strand, within BPHYT_RS24445

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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1,048,458 + BPHYT_RS24435 0.14 +0.0
1,048,459 - BPHYT_RS24435 0.14 +1.0
1,048,499 - BPHYT_RS24435 0.18 -0.4
1,048,691 + BPHYT_RS24435 0.38 +0.2
1,048,691 + BPHYT_RS24435 0.38 -0.2
1,048,691 + BPHYT_RS24435 0.38 +0.6
1,048,692 - BPHYT_RS24435 0.38 -0.5
1,048,697 + BPHYT_RS24435 0.39 -2.3
1,048,716 + BPHYT_RS24435 0.41 -3.0
1,048,774 + BPHYT_RS24435 0.47 +0.7
1,048,938 + BPHYT_RS24435 0.64 -0.1
1,049,450 - BPHYT_RS24440 0.16 -2.2
1,049,807 + BPHYT_RS24440 0.52 -1.6
1,050,054 - BPHYT_RS24440 0.77 -3.8
1,050,054 - BPHYT_RS24440 0.77 +0.1
1,051,105 - BPHYT_RS24445 0.21 +0.3

Or see this region's nucleotide sequence