Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24335 and BPHYT_RS24340 are separated by 2 nucleotides BPHYT_RS24340 and BPHYT_RS24345 are separated by 282 nucleotides BPHYT_RS24345 and BPHYT_RS24350 are separated by 45 nucleotides BPHYT_RS24350 and BPHYT_RS24355 are separated by 99 nucleotides
BPHYT_RS24335: BPHYT_RS24335 - toxin HipA, at 1,025,758 to 1,027,098
_RS24335
BPHYT_RS24340: BPHYT_RS24340 - XRE family transcriptional regulator, at 1,027,101 to 1,027,355
_RS24340
BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978
_RS24345
BPHYT_RS24350: BPHYT_RS24350 - hypothetical protein, at 1,029,024 to 1,029,656
_RS24350
BPHYT_RS24355: BPHYT_RS24355 - hypothetical protein, at 1,029,756 to 1,030,007
_RS24355
Position (kb)
1027
1028
1029 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1026.840 kb on - strand, within BPHYT_RS24335 at 1026.999 kb on - strand at 1027.483 kb on - strand at 1027.502 kb on - strand at 1027.622 kb on - strand at 1027.622 kb on - strand at 1027.749 kb on + strand at 1027.752 kb on + strand at 1027.752 kb on + strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1027.753 kb on - strand at 1029.060 kb on + strand at 1029.060 kb on + strand at 1029.330 kb on + strand, within BPHYT_RS24350 at 1029.585 kb on + strand, within BPHYT_RS24350 at 1029.586 kb on - strand, within BPHYT_RS24350 at 1029.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 1,026,840 - BPHYT_RS24335 0.81 +0.5 1,026,999 - -2.3 1,027,483 - +0.3 1,027,502 - -0.5 1,027,622 - -1.5 1,027,622 - -0.8 1,027,749 + -0.4 1,027,752 + +1.7 1,027,752 + +0.1 1,027,753 - +1.7 1,027,753 - +0.2 1,027,753 - -0.2 1,027,753 - +0.2 1,029,060 + -3.2 1,029,060 + -2.6 1,029,330 + BPHYT_RS24350 0.48 +0.5 1,029,585 + BPHYT_RS24350 0.89 +3.1 1,029,586 - BPHYT_RS24350 0.89 +1.1 1,029,768 + -2.4
Or see this region's nucleotide sequence