Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20870

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20865 and BPHYT_RS20870 are separated by 688 nucleotidesBPHYT_RS20870 and BPHYT_RS20875 are separated by 523 nucleotides BPHYT_RS20865: BPHYT_RS20865 - 2-hydroxyacid dehydrogenase, at 242,720 to 243,685 _RS20865 BPHYT_RS20870: BPHYT_RS20870 - cysteine hydrolase, at 244,374 to 244,943 _RS20870 BPHYT_RS20875: BPHYT_RS20875 - hypothetical protein, at 245,467 to 245,748 _RS20875 Position (kb) 244 245Strain fitness (log2 ratio) -1 0 1at 243.424 kb on + strand, within BPHYT_RS20865at 243.424 kb on + strand, within BPHYT_RS20865at 243.916 kb on + strandat 244.052 kb on + strandat 244.375 kb on + strandat 244.444 kb on - strand, within BPHYT_RS20870at 244.475 kb on - strand, within BPHYT_RS20870at 244.475 kb on - strand, within BPHYT_RS20870at 244.870 kb on - strand, within BPHYT_RS20870

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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243,424 + BPHYT_RS20865 0.73 -0.4
243,424 + BPHYT_RS20865 0.73 +0.1
243,916 + +0.2
244,052 + -0.2
244,375 + -0.9
244,444 - BPHYT_RS20870 0.12 +0.2
244,475 - BPHYT_RS20870 0.18 -0.9
244,475 - BPHYT_RS20870 0.18 -0.6
244,870 - BPHYT_RS20870 0.87 -0.6

Or see this region's nucleotide sequence