Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20795

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20790 and BPHYT_RS20795 are separated by 246 nucleotidesBPHYT_RS20795 and BPHYT_RS20800 are separated by 95 nucleotides BPHYT_RS20790: BPHYT_RS20790 - TetR family transcriptional regulator, at 226,144 to 226,794 _RS20790 BPHYT_RS20795: BPHYT_RS20795 - sorbosone dehydrogenase, at 227,041 to 228,549 _RS20795 BPHYT_RS20800: BPHYT_RS20800 - alanine-phosphoribitol ligase, at 228,645 to 230,318 _RS20800 Position (kb) 227 228 229Strain fitness (log2 ratio) -3 -2 -1 0 1at 226.571 kb on + strand, within BPHYT_RS20790at 226.625 kb on + strand, within BPHYT_RS20790at 226.897 kb on - strandat 227.425 kb on + strand, within BPHYT_RS20795at 227.634 kb on + strand, within BPHYT_RS20795at 227.635 kb on - strand, within BPHYT_RS20795at 227.742 kb on + strand, within BPHYT_RS20795at 227.742 kb on + strand, within BPHYT_RS20795at 227.743 kb on - strand, within BPHYT_RS20795at 228.270 kb on + strand, within BPHYT_RS20795at 228.491 kb on - strandat 228.491 kb on - strandat 228.622 kb on - strandat 228.648 kb on + strandat 228.648 kb on + strandat 228.648 kb on + strandat 228.648 kb on + strandat 228.654 kb on + strandat 228.654 kb on + strandat 228.765 kb on + strandat 228.767 kb on + strandat 228.943 kb on - strand, within BPHYT_RS20800at 229.143 kb on + strand, within BPHYT_RS20800at 229.143 kb on + strand, within BPHYT_RS20800

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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226,571 + BPHYT_RS20790 0.66 -2.2
226,625 + BPHYT_RS20790 0.74 -1.0
226,897 - +0.5
227,425 + BPHYT_RS20795 0.25 +0.7
227,634 + BPHYT_RS20795 0.39 +0.7
227,635 - BPHYT_RS20795 0.39 -0.0
227,742 + BPHYT_RS20795 0.46 +0.4
227,742 + BPHYT_RS20795 0.46 +0.6
227,743 - BPHYT_RS20795 0.47 -1.2
228,270 + BPHYT_RS20795 0.81 +1.5
228,491 - -3.0
228,491 - +1.2
228,622 - +0.2
228,648 + -0.2
228,648 + +0.2
228,648 + +1.0
228,648 + +0.2
228,654 + +1.2
228,654 + -1.0
228,765 + +1.2
228,767 + +1.2
228,943 - BPHYT_RS20800 0.18 -0.4
229,143 + BPHYT_RS20800 0.30 +1.7
229,143 + BPHYT_RS20800 0.30 +0.0

Or see this region's nucleotide sequence