Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18640 and BPHYT_RS18645 overlap by 4 nucleotides BPHYT_RS18645 and BPHYT_RS18650 are separated by 213 nucleotides
BPHYT_RS18640: BPHYT_RS18640 - membrane protein, at 4,191,905 to 4,192,654
_RS18640
BPHYT_RS18645: BPHYT_RS18645 - membrane protein, at 4,192,651 to 4,193,607
_RS18645
BPHYT_RS18650: BPHYT_RS18650 - hypothetical protein, at 4,193,821 to 4,195,629
_RS18650
Position (kb)
4192
4193
4194 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.774 kb on - strand at 4191.785 kb on + strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.925 kb on - strand at 4191.969 kb on + strand at 4191.969 kb on + strand at 4192.088 kb on - strand, within BPHYT_RS18640 at 4192.214 kb on + strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand, within BPHYT_RS18640 at 4192.215 kb on - strand at 4192.669 kb on + strand at 4192.670 kb on - strand at 4192.670 kb on - strand at 4192.775 kb on - strand, within BPHYT_RS18645 at 4192.829 kb on + strand, within BPHYT_RS18645 at 4192.830 kb on - strand, within BPHYT_RS18645 at 4193.255 kb on - strand, within BPHYT_RS18645 at 4193.255 kb on - strand, within BPHYT_RS18645 at 4193.294 kb on - strand, within BPHYT_RS18645 at 4193.444 kb on - strand, within BPHYT_RS18645 at 4193.574 kb on + strand at 4193.604 kb on - strand at 4193.605 kb on + strand at 4193.663 kb on - strand at 4193.709 kb on - strand at 4193.709 kb on - strand at 4193.746 kb on + strand at 4193.747 kb on - strand at 4193.747 kb on - strand at 4193.875 kb on + strand at 4193.875 kb on + strand at 4193.876 kb on - strand at 4193.979 kb on + strand at 4194.152 kb on - strand, within BPHYT_RS18650 at 4194.253 kb on + strand, within BPHYT_RS18650
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 4,191,774 - -0.9 4,191,774 - +0.8 4,191,774 - +0.1 4,191,774 - -0.1 4,191,785 + +1.6 4,191,925 - -0.3 4,191,925 - +0.3 4,191,925 - -0.1 4,191,969 + +0.7 4,191,969 + +0.5 4,192,088 - BPHYT_RS18640 0.24 +0.8 4,192,214 + BPHYT_RS18640 0.41 -3.0 4,192,215 - BPHYT_RS18640 0.41 -0.0 4,192,215 - BPHYT_RS18640 0.41 +1.1 4,192,215 - +1.3 4,192,669 + +0.4 4,192,670 - +0.2 4,192,670 - -0.3 4,192,775 - BPHYT_RS18645 0.13 +0.6 4,192,829 + BPHYT_RS18645 0.19 +1.0 4,192,830 - BPHYT_RS18645 0.19 +0.8 4,193,255 - BPHYT_RS18645 0.63 -0.5 4,193,255 - BPHYT_RS18645 0.63 -1.9 4,193,294 - BPHYT_RS18645 0.67 -0.3 4,193,444 - BPHYT_RS18645 0.83 -2.2 4,193,574 + +0.7 4,193,604 - -0.1 4,193,605 + -0.8 4,193,663 - -0.4 4,193,709 - +0.6 4,193,709 - +0.1 4,193,746 + +0.4 4,193,747 - -0.2 4,193,747 - +0.2 4,193,875 + +0.1 4,193,875 + +0.8 4,193,876 - +0.7 4,193,979 + +0.2 4,194,152 - BPHYT_RS18650 0.18 -0.1 4,194,253 + BPHYT_RS18650 0.24 -0.0
Or see this region's nucleotide sequence