Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16640

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16630 and BPHYT_RS16635 are separated by 104 nucleotidesBPHYT_RS16635 and BPHYT_RS16640 are separated by 31 nucleotidesBPHYT_RS16640 and BPHYT_RS16645 are separated by 206 nucleotides BPHYT_RS16630: BPHYT_RS16630 - phosphoglycerate mutase, at 3,767,588 to 3,768,259 _RS16630 BPHYT_RS16635: BPHYT_RS16635 - D-aminoacyl-tRNA deacylase, at 3,768,364 to 3,768,822 _RS16635 BPHYT_RS16640: BPHYT_RS16640 - tyrosyl-tRNA synthetase, at 3,768,854 to 3,770,104 _RS16640 BPHYT_RS16645: BPHYT_RS16645 - anhydro-N-acetylmuramic acid kinase, at 3,770,311 to 3,771,441 _RS16645 Position (kb) 3768 3769 3770 3771Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3767.866 kb on - strand, within BPHYT_RS16630at 3767.866 kb on - strand, within BPHYT_RS16630at 3767.866 kb on - strand, within BPHYT_RS16630at 3767.932 kb on - strand, within BPHYT_RS16630at 3767.932 kb on - strand, within BPHYT_RS16630at 3767.932 kb on - strand, within BPHYT_RS16630at 3767.965 kb on + strand, within BPHYT_RS16630at 3767.989 kb on - strand, within BPHYT_RS16630at 3770.145 kb on - strandat 3770.145 kb on - strandat 3770.145 kb on - strandat 3770.243 kb on - strandat 3770.264 kb on + strandat 3770.265 kb on - strandat 3770.272 kb on + strandat 3770.272 kb on + strandat 3770.542 kb on + strand, within BPHYT_RS16645at 3770.650 kb on + strand, within BPHYT_RS16645at 3770.651 kb on - strand, within BPHYT_RS16645at 3770.651 kb on - strand, within BPHYT_RS16645at 3770.651 kb on - strand, within BPHYT_RS16645at 3770.653 kb on - strand, within BPHYT_RS16645at 3770.698 kb on - strand, within BPHYT_RS16645at 3770.839 kb on - strand, within BPHYT_RS16645

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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3,767,866 - BPHYT_RS16630 0.41 +0.3
3,767,866 - BPHYT_RS16630 0.41 -0.4
3,767,866 - BPHYT_RS16630 0.41 +0.5
3,767,932 - BPHYT_RS16630 0.51 +0.2
3,767,932 - BPHYT_RS16630 0.51 -0.0
3,767,932 - BPHYT_RS16630 0.51 +0.4
3,767,965 + BPHYT_RS16630 0.56 +0.4
3,767,989 - BPHYT_RS16630 0.60 +2.9
3,770,145 - -1.2
3,770,145 - -0.7
3,770,145 - -0.3
3,770,243 - -0.4
3,770,264 + +0.8
3,770,265 - -0.4
3,770,272 + +1.6
3,770,272 + +1.5
3,770,542 + BPHYT_RS16645 0.20 -0.9
3,770,650 + BPHYT_RS16645 0.30 -3.3
3,770,651 - BPHYT_RS16645 0.30 -0.8
3,770,651 - BPHYT_RS16645 0.30 -2.9
3,770,651 - BPHYT_RS16645 0.30 -0.3
3,770,653 - BPHYT_RS16645 0.30 -0.7
3,770,698 - BPHYT_RS16645 0.34 +0.6
3,770,839 - BPHYT_RS16645 0.47 -0.2

Or see this region's nucleotide sequence