Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06850 and BPHYT_RS06855 are separated by 120 nucleotides BPHYT_RS06855 and BPHYT_RS06860 are separated by 11 nucleotides BPHYT_RS06860 and BPHYT_RS06865 are separated by 123 nucleotides
BPHYT_RS06850: BPHYT_RS06850 - DNA polymerase III subunit alpha, at 1,549,762 to 1,553,343
_RS06850
BPHYT_RS06855: BPHYT_RS06855 - dTDP-4-dehydrorhamnose reductase, at 1,553,464 to 1,554,384
_RS06855
BPHYT_RS06860: BPHYT_RS06860 - heavy metal transporter, at 1,554,396 to 1,554,635
_RS06860
BPHYT_RS06865: BPHYT_RS06865 - MerR family transcriptional regulator, at 1,554,759 to 1,555,181
_RS06865
Position (kb)
1553
1554
1555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1552.546 kb on + strand, within BPHYT_RS06850 at 1552.546 kb on + strand, within BPHYT_RS06850 at 1552.830 kb on - strand, within BPHYT_RS06850 at 1553.155 kb on + strand at 1553.380 kb on + strand at 1553.380 kb on + strand at 1553.381 kb on - strand at 1553.381 kb on - strand at 1553.465 kb on + strand at 1553.465 kb on + strand at 1553.465 kb on + strand at 1553.466 kb on - strand at 1553.466 kb on - strand at 1553.542 kb on + strand at 1553.584 kb on - strand, within BPHYT_RS06855 at 1553.584 kb on - strand, within BPHYT_RS06855 at 1553.614 kb on - strand, within BPHYT_RS06855 at 1553.675 kb on + strand, within BPHYT_RS06855 at 1553.676 kb on - strand, within BPHYT_RS06855 at 1553.762 kb on - strand, within BPHYT_RS06855 at 1553.979 kb on + strand, within BPHYT_RS06855 at 1553.979 kb on + strand, within BPHYT_RS06855 at 1553.979 kb on + strand, within BPHYT_RS06855 at 1553.979 kb on + strand, within BPHYT_RS06855 at 1553.980 kb on - strand, within BPHYT_RS06855 at 1553.980 kb on - strand, within BPHYT_RS06855 at 1553.988 kb on - strand, within BPHYT_RS06855 at 1554.023 kb on + strand, within BPHYT_RS06855 at 1554.051 kb on - strand, within BPHYT_RS06855 at 1554.057 kb on + strand, within BPHYT_RS06855 at 1554.615 kb on + strand at 1554.616 kb on - strand at 1554.676 kb on + strand at 1554.676 kb on + strand at 1554.676 kb on + strand at 1554.677 kb on - strand at 1554.728 kb on + strand at 1554.728 kb on + strand at 1554.729 kb on - strand at 1554.816 kb on + strand, within BPHYT_RS06865 at 1554.816 kb on + strand, within BPHYT_RS06865 at 1554.817 kb on - strand, within BPHYT_RS06865 at 1554.871 kb on + strand, within BPHYT_RS06865 at 1555.258 kb on + strand at 1555.311 kb on + strand at 1555.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 1,552,546 + BPHYT_RS06850 0.78 -0.2 1,552,546 + BPHYT_RS06850 0.78 +0.3 1,552,830 - BPHYT_RS06850 0.86 +0.6 1,553,155 + +0.6 1,553,380 + -0.2 1,553,380 + +1.1 1,553,381 - -0.5 1,553,381 - -1.5 1,553,465 + -1.6 1,553,465 + -0.3 1,553,465 + +0.6 1,553,466 - -1.6 1,553,466 - +0.5 1,553,542 + +0.4 1,553,584 - BPHYT_RS06855 0.13 -3.0 1,553,584 - BPHYT_RS06855 0.13 -0.4 1,553,614 - BPHYT_RS06855 0.16 +0.6 1,553,675 + BPHYT_RS06855 0.23 +0.5 1,553,676 - BPHYT_RS06855 0.23 +1.2 1,553,762 - BPHYT_RS06855 0.32 +1.6 1,553,979 + BPHYT_RS06855 0.56 +0.5 1,553,979 + BPHYT_RS06855 0.56 +0.3 1,553,979 + BPHYT_RS06855 0.56 -2.0 1,553,979 + BPHYT_RS06855 0.56 +0.2 1,553,980 - BPHYT_RS06855 0.56 +2.0 1,553,980 - BPHYT_RS06855 0.56 +0.4 1,553,988 - BPHYT_RS06855 0.57 -1.8 1,554,023 + BPHYT_RS06855 0.61 -0.2 1,554,051 - BPHYT_RS06855 0.64 +1.8 1,554,057 + BPHYT_RS06855 0.64 +0.5 1,554,615 + +0.1 1,554,616 - +0.1 1,554,676 + -0.0 1,554,676 + -1.5 1,554,676 + +0.4 1,554,677 - -0.0 1,554,728 + -0.5 1,554,728 + +2.3 1,554,729 - +1.1 1,554,816 + BPHYT_RS06865 0.13 -3.5 1,554,816 + BPHYT_RS06865 0.13 -1.6 1,554,817 - BPHYT_RS06865 0.14 +0.6 1,554,871 + BPHYT_RS06865 0.26 -0.2 1,555,258 + +0.2 1,555,311 + +0.1 1,555,325 - -0.0
Or see this region's nucleotide sequence