Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03720 and BPHYT_RS03725 are separated by 516 nucleotides BPHYT_RS03725 and BPHYT_RS03730 are separated by 6 nucleotides BPHYT_RS03730 and BPHYT_RS03735 are separated by 198 nucleotides
BPHYT_RS03720: BPHYT_RS03720 - malonic semialdehyde reductase, at 844,006 to 844,752
_RS03720
BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516
_RS03725
BPHYT_RS03730: BPHYT_RS03730 - transcriptional repressor NrdR, at 846,523 to 847,005
_RS03730
BPHYT_RS03735: BPHYT_RS03735 - pilus assembly protein, at 847,204 to 847,770
_RS03735
Position (kb) 845
846
847 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 844.415 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.602 kb on - strand, within BPHYT_RS03720 at 844.903 kb on + strand at 844.903 kb on + strand at 844.903 kb on + strand at 845.015 kb on + strand at 845.207 kb on + strand at 845.214 kb on + strand at 845.268 kb on + strand at 845.269 kb on - strand at 845.857 kb on + strand, within BPHYT_RS03725 at 846.644 kb on - strand, within BPHYT_RS03730 at 846.800 kb on - strand, within BPHYT_RS03730 at 846.893 kb on - strand, within BPHYT_RS03730 at 847.385 kb on - strand at 847.385 kb on - strand, within BPHYT_RS03735 at 847.385 kb on - strand, within BPHYT_RS03735
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 844,415 - BPHYT_RS03720 0.55 +0.1 844,602 - BPHYT_RS03720 0.80 +0.7 844,602 - BPHYT_RS03720 0.80 -0.4 844,903 + +1.7 844,903 + -1.4 844,903 + +1.1 845,015 + -1.0 845,207 + +1.2 845,214 + -0.6 845,268 + -0.5 845,269 - -2.2 845,857 + BPHYT_RS03725 0.47 -3.5 846,644 - BPHYT_RS03730 0.25 -1.6 846,800 - BPHYT_RS03730 0.57 -1.2 846,893 - BPHYT_RS03730 0.77 +0.2 847,385 - -0.2 847,385 - BPHYT_RS03735 0.32 -0.2 847,385 - BPHYT_RS03735 0.32 +0.2
Or see this region's nucleotide sequence