Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01640 and BPHYT_RS01645 are separated by 677 nucleotides BPHYT_RS01645 and BPHYT_RS01650 are separated by 190 nucleotides
BPHYT_RS01640: BPHYT_RS01640 - molecular chaperone DnaK, at 372,494 to 372,913
_RS01640
BPHYT_RS01645: BPHYT_RS01645 - cobalamin biosynthesis protein CobW, at 373,591 to 374,679
_RS01645
BPHYT_RS01650: BPHYT_RS01650 - 23S rRNA methyltransferase, at 374,870 to 376,072
_RS01650
Position (kb) 373
374
375 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 372.948 kb on - strand at 372.948 kb on - strand at 372.952 kb on - strand at 372.952 kb on - strand at 372.952 kb on - strand at 373.077 kb on - strand at 373.228 kb on - strand at 373.272 kb on - strand at 373.272 kb on - strand at 373.272 kb on - strand at 373.277 kb on - strand at 373.277 kb on - strand at 373.277 kb on - strand at 373.293 kb on - strand at 373.293 kb on - strand at 373.320 kb on - strand at 373.333 kb on - strand at 373.344 kb on - strand at 373.386 kb on - strand at 373.386 kb on - strand at 373.386 kb on - strand at 373.451 kb on - strand at 373.495 kb on + strand at 373.496 kb on - strand at 373.496 kb on - strand at 373.650 kb on + strand at 373.763 kb on + strand, within BPHYT_RS01645 at 373.764 kb on - strand, within BPHYT_RS01645 at 373.776 kb on + strand, within BPHYT_RS01645 at 373.778 kb on + strand, within BPHYT_RS01645 at 373.778 kb on + strand, within BPHYT_RS01645 at 373.779 kb on - strand, within BPHYT_RS01645 at 373.779 kb on - strand, within BPHYT_RS01645 at 373.799 kb on + strand, within BPHYT_RS01645 at 373.800 kb on - strand, within BPHYT_RS01645 at 373.800 kb on - strand, within BPHYT_RS01645 at 373.981 kb on - strand, within BPHYT_RS01645 at 374.692 kb on + strand at 374.693 kb on - strand at 374.693 kb on - strand at 374.693 kb on - strand at 374.693 kb on - strand at 374.702 kb on + strand at 374.703 kb on - strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.780 kb on + strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.781 kb on - strand at 374.849 kb on + strand at 374.849 kb on + strand at 374.861 kb on + strand at 374.901 kb on + strand at 374.901 kb on + strand at 375.108 kb on + strand, within BPHYT_RS01650 at 375.109 kb on - strand, within BPHYT_RS01650 at 375.109 kb on - strand, within BPHYT_RS01650 at 375.109 kb on - strand, within BPHYT_RS01650 at 375.373 kb on - strand, within BPHYT_RS01650 at 375.447 kb on + strand, within BPHYT_RS01650 at 375.447 kb on + strand, within BPHYT_RS01650 at 375.447 kb on + strand, within BPHYT_RS01650 at 375.447 kb on + strand, within BPHYT_RS01650 at 375.447 kb on + strand, within BPHYT_RS01650 at 375.448 kb on - strand, within BPHYT_RS01650
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 372,948 - +0.3 372,948 - -0.1 372,952 - +2.3 372,952 - +0.2 372,952 - +0.3 373,077 - +0.3 373,228 - +0.8 373,272 - -0.8 373,272 - -0.1 373,272 - -0.2 373,277 - +0.1 373,277 - +0.7 373,277 - -0.1 373,293 - +0.3 373,293 - +0.7 373,320 - +0.0 373,333 - -0.7 373,344 - -2.0 373,386 - +0.7 373,386 - +0.3 373,386 - -0.3 373,451 - +1.7 373,495 + +0.3 373,496 - -0.8 373,496 - -0.5 373,650 + -0.5 373,763 + BPHYT_RS01645 0.16 +1.3 373,764 - BPHYT_RS01645 0.16 +0.4 373,776 + BPHYT_RS01645 0.17 -0.2 373,778 + BPHYT_RS01645 0.17 -0.9 373,778 + BPHYT_RS01645 0.17 +0.3 373,779 - BPHYT_RS01645 0.17 -0.1 373,779 - BPHYT_RS01645 0.17 +0.5 373,799 + BPHYT_RS01645 0.19 -0.6 373,800 - BPHYT_RS01645 0.19 +0.1 373,800 - BPHYT_RS01645 0.19 +0.5 373,981 - BPHYT_RS01645 0.36 -0.4 374,692 + -0.1 374,693 - -4.1 374,693 - +0.2 374,693 - +0.7 374,693 - +0.6 374,702 + -0.4 374,703 - +0.2 374,780 + -0.4 374,780 + -1.1 374,780 + +0.1 374,780 + +0.6 374,780 + -0.7 374,780 + -1.1 374,780 + -0.1 374,780 + +0.1 374,780 + -1.8 374,780 + -0.8 374,780 + +0.1 374,780 + +1.8 374,780 + -1.3 374,781 - -0.5 374,781 - +0.7 374,781 - +0.9 374,781 - +0.5 374,781 - +0.1 374,781 - -0.3 374,781 - -0.3 374,781 - +0.2 374,781 - -0.2 374,781 - -0.6 374,849 + +0.1 374,849 + -2.6 374,861 + -0.5 374,901 + +0.4 374,901 + -2.3 375,108 + BPHYT_RS01650 0.20 +0.2 375,109 - BPHYT_RS01650 0.20 +0.4 375,109 - BPHYT_RS01650 0.20 -0.5 375,109 - BPHYT_RS01650 0.20 +1.2 375,373 - BPHYT_RS01650 0.42 -1.1 375,447 + BPHYT_RS01650 0.48 +0.4 375,447 + BPHYT_RS01650 0.48 +0.6 375,447 + BPHYT_RS01650 0.48 -0.3 375,447 + BPHYT_RS01650 0.48 +0.0 375,447 + BPHYT_RS01650 0.48 +0.1 375,448 - BPHYT_RS01650 0.48 -0.3
Or see this region's nucleotide sequence