Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23740 and BPHYT_RS23745 are separated by 277 nucleotides BPHYT_RS23745 and BPHYT_RS23750 are separated by 202 nucleotides BPHYT_RS23750 and BPHYT_RS23755 are separated by 125 nucleotides
BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792
_RS23740
BPHYT_RS23745: BPHYT_RS23745 - hypothetical protein, at 895,070 to 895,306
_RS23745
BPHYT_RS23750: BPHYT_RS23750 - phosphonate ABC transporter substrate-binding protein, at 895,509 to 896,597
_RS23750
BPHYT_RS23755: BPHYT_RS23755 - phosphonate ABC transporter ATP-binding protein, at 896,723 to 897,823
_RS23755
Position (kb) 895
896
897 Strain fitness (log2 ratio) -2
-1
0
1 at 894.583 kb on + strand, within BPHYT_RS23740 at 894.835 kb on - strand at 894.939 kb on - strand at 894.981 kb on + strand at 895.040 kb on - strand at 895.040 kb on - strand at 895.040 kb on - strand at 895.061 kb on - strand at 895.305 kb on - strand at 895.635 kb on + strand, within BPHYT_RS23750 at 895.635 kb on + strand, within BPHYT_RS23750 at 895.662 kb on + strand, within BPHYT_RS23750 at 895.843 kb on - strand, within BPHYT_RS23750 at 895.857 kb on + strand, within BPHYT_RS23750 at 895.858 kb on - strand, within BPHYT_RS23750 at 895.920 kb on + strand, within BPHYT_RS23750 at 895.921 kb on - strand, within BPHYT_RS23750 at 896.148 kb on + strand, within BPHYT_RS23750 at 896.595 kb on + strand at 896.699 kb on + strand at 896.699 kb on + strand at 897.056 kb on + strand, within BPHYT_RS23755 at 897.056 kb on + strand, within BPHYT_RS23755 at 897.056 kb on + strand, within BPHYT_RS23755 at 897.057 kb on - strand, within BPHYT_RS23755 at 897.309 kb on + strand, within BPHYT_RS23755 at 897.309 kb on + strand, within BPHYT_RS23755 at 897.310 kb on - strand, within BPHYT_RS23755 at 897.310 kb on - strand, within BPHYT_RS23755 at 897.474 kb on - strand, within BPHYT_RS23755 at 897.566 kb on + strand, within BPHYT_RS23755
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 894,583 + BPHYT_RS23740 0.72 -1.3 894,835 - +1.2 894,939 - -2.4 894,981 + -0.4 895,040 - +0.1 895,040 - -0.8 895,040 - +0.6 895,061 - -0.0 895,305 - -1.0 895,635 + BPHYT_RS23750 0.12 +0.2 895,635 + BPHYT_RS23750 0.12 +0.3 895,662 + BPHYT_RS23750 0.14 -1.4 895,843 - BPHYT_RS23750 0.31 -0.7 895,857 + BPHYT_RS23750 0.32 +0.5 895,858 - BPHYT_RS23750 0.32 -0.8 895,920 + BPHYT_RS23750 0.38 +0.1 895,921 - BPHYT_RS23750 0.38 +0.7 896,148 + BPHYT_RS23750 0.59 -0.7 896,595 + +0.6 896,699 + -0.1 896,699 + -0.6 897,056 + BPHYT_RS23755 0.30 +0.7 897,056 + BPHYT_RS23755 0.30 -0.4 897,056 + BPHYT_RS23755 0.30 +1.6 897,057 - BPHYT_RS23755 0.30 +0.1 897,309 + BPHYT_RS23755 0.53 +0.1 897,309 + BPHYT_RS23755 0.53 +0.1 897,310 - BPHYT_RS23755 0.53 -0.2 897,310 - BPHYT_RS23755 0.53 +1.0 897,474 - BPHYT_RS23755 0.68 +0.1 897,566 + BPHYT_RS23755 0.77 +0.1
Or see this region's nucleotide sequence