Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10910

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10905 and BPHYT_RS10910 are separated by 96 nucleotidesBPHYT_RS10910 and BPHYT_RS10915 overlap by 4 nucleotides BPHYT_RS10905: BPHYT_RS10905 - membrane protein, at 2,481,947 to 2,482,942 _RS10905 BPHYT_RS10910: BPHYT_RS10910 - glucosyltransferase, at 2,483,039 to 2,484,199 _RS10910 BPHYT_RS10915: BPHYT_RS10915 - radical SAM protein, at 2,484,196 to 2,485,617 _RS10915 Position (kb) 2483 2484 2485Strain fitness (log2 ratio) -1 0 1 2 3at 2482.694 kb on - strand, within BPHYT_RS10905at 2482.989 kb on + strandat 2483.030 kb on + strandat 2483.698 kb on + strand, within BPHYT_RS10910at 2483.748 kb on + strand, within BPHYT_RS10910at 2483.748 kb on + strand, within BPHYT_RS10910at 2483.977 kb on - strand, within BPHYT_RS10910at 2484.163 kb on - strandat 2484.329 kb on + strandat 2484.330 kb on - strandat 2484.330 kb on - strandat 2484.947 kb on + strand, within BPHYT_RS10915at 2485.028 kb on + strand, within BPHYT_RS10915at 2485.029 kb on - strand, within BPHYT_RS10915

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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2,482,694 - BPHYT_RS10905 0.75 +3.2
2,482,989 + +1.1
2,483,030 + +0.2
2,483,698 + BPHYT_RS10910 0.57 +1.8
2,483,748 + BPHYT_RS10910 0.61 +0.5
2,483,748 + BPHYT_RS10910 0.61 +2.2
2,483,977 - BPHYT_RS10910 0.81 -0.8
2,484,163 - -0.0
2,484,329 + +0.1
2,484,330 - +1.0
2,484,330 - -0.0
2,484,947 + BPHYT_RS10915 0.53 +1.3
2,485,028 + BPHYT_RS10915 0.59 -1.3
2,485,029 - BPHYT_RS10915 0.59 -0.4

Or see this region's nucleotide sequence