Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06375

Experiment: LB_DAP agar plate, outgrowth in LB_gent

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06370 and BPHYT_RS06375 are separated by 526 nucleotidesBPHYT_RS06375 and BPHYT_RS06380 are separated by 9 nucleotides BPHYT_RS06370: BPHYT_RS06370 - carboxylesterase, at 1,450,453 to 1,451,454 _RS06370 BPHYT_RS06375: BPHYT_RS06375 - ribonuclease, at 1,451,981 to 1,453,450 _RS06375 BPHYT_RS06380: BPHYT_RS06380 - septum formation protein Maf, at 1,453,460 to 1,454,089 _RS06380 Position (kb) 1451 1452 1453 1454Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1451.058 kb on + strand, within BPHYT_RS06370at 1451.078 kb on + strand, within BPHYT_RS06370at 1451.079 kb on - strand, within BPHYT_RS06370at 1451.129 kb on + strand, within BPHYT_RS06370at 1451.203 kb on + strand, within BPHYT_RS06370at 1451.527 kb on + strandat 1451.615 kb on - strandat 1451.615 kb on - strandat 1451.615 kb on - strandat 1451.637 kb on - strandat 1451.637 kb on - strandat 1451.790 kb on - strandat 1451.790 kb on - strandat 1451.914 kb on + strandat 1451.916 kb on + strandat 1451.917 kb on - strandat 1451.995 kb on + strandat 1452.000 kb on + strandat 1452.052 kb on + strandat 1452.187 kb on - strand, within BPHYT_RS06375at 1452.416 kb on - strand, within BPHYT_RS06375at 1452.523 kb on - strand, within BPHYT_RS06375at 1452.580 kb on - strand, within BPHYT_RS06375at 1452.672 kb on + strand, within BPHYT_RS06375at 1452.672 kb on + strand, within BPHYT_RS06375at 1452.673 kb on - strand, within BPHYT_RS06375at 1452.736 kb on - strand, within BPHYT_RS06375at 1452.822 kb on + strand, within BPHYT_RS06375at 1452.948 kb on + strand, within BPHYT_RS06375at 1453.461 kb on + strandat 1453.522 kb on + strandat 1453.531 kb on - strand, within BPHYT_RS06380at 1453.531 kb on - strand, within BPHYT_RS06380at 1453.543 kb on + strand, within BPHYT_RS06380at 1453.543 kb on + strand, within BPHYT_RS06380at 1453.581 kb on + strand, within BPHYT_RS06380at 1453.825 kb on + strand, within BPHYT_RS06380at 1453.918 kb on - strand, within BPHYT_RS06380at 1454.053 kb on - strandat 1454.218 kb on + strandat 1454.274 kb on - strandat 1454.313 kb on + strandat 1454.313 kb on + strandat 1454.314 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent
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1,451,058 + BPHYT_RS06370 0.60 +0.2
1,451,078 + BPHYT_RS06370 0.62 -1.6
1,451,079 - BPHYT_RS06370 0.62 +0.4
1,451,129 + BPHYT_RS06370 0.67 -0.0
1,451,203 + BPHYT_RS06370 0.75 -0.6
1,451,527 + +1.1
1,451,615 - +0.4
1,451,615 - +0.2
1,451,615 - +0.5
1,451,637 - -0.7
1,451,637 - +0.1
1,451,790 - -1.2
1,451,790 - +0.7
1,451,914 + +0.2
1,451,916 + -0.7
1,451,917 - +0.7
1,451,995 + -0.4
1,452,000 + -1.3
1,452,052 + -3.0
1,452,187 - BPHYT_RS06375 0.14 -1.6
1,452,416 - BPHYT_RS06375 0.30 +1.0
1,452,523 - BPHYT_RS06375 0.37 -0.8
1,452,580 - BPHYT_RS06375 0.41 +0.4
1,452,672 + BPHYT_RS06375 0.47 +0.3
1,452,672 + BPHYT_RS06375 0.47 -1.0
1,452,673 - BPHYT_RS06375 0.47 -0.2
1,452,736 - BPHYT_RS06375 0.51 -1.0
1,452,822 + BPHYT_RS06375 0.57 -1.4
1,452,948 + BPHYT_RS06375 0.66 -0.6
1,453,461 + -0.4
1,453,522 + +0.0
1,453,531 - BPHYT_RS06380 0.11 +0.4
1,453,531 - BPHYT_RS06380 0.11 -1.2
1,453,543 + BPHYT_RS06380 0.13 +2.6
1,453,543 + BPHYT_RS06380 0.13 +0.6
1,453,581 + BPHYT_RS06380 0.19 +0.9
1,453,825 + BPHYT_RS06380 0.58 -0.2
1,453,918 - BPHYT_RS06380 0.73 -3.4
1,454,053 - -1.6
1,454,218 + +0.2
1,454,274 - -1.1
1,454,313 + +0.3
1,454,313 + +0.3
1,454,314 - +0.9

Or see this region's nucleotide sequence