Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03605 and BPHYT_RS03610 are separated by 39 nucleotides BPHYT_RS03610 and BPHYT_RS03615 overlap by 4 nucleotides BPHYT_RS03615 and BPHYT_RS03620 are separated by 48 nucleotides
BPHYT_RS03605: BPHYT_RS03605 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 820,505 to 821,320
_RS03605
BPHYT_RS03610: BPHYT_RS03610 - deoxyadenosine kinase, at 821,360 to 822,040
_RS03610
BPHYT_RS03615: BPHYT_RS03615 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 822,037 to 822,585
_RS03615
BPHYT_RS03620: BPHYT_RS03620 - poly(A) polymerase, at 822,634 to 824,223
_RS03620
Position (kb) 821
822
823 Strain fitness (log2 ratio) -2
-1
0
1 at 820.417 kb on - strand at 820.417 kb on - strand at 820.417 kb on - strand at 820.514 kb on - strand at 821.484 kb on - strand, within BPHYT_RS03610 at 821.500 kb on - strand, within BPHYT_RS03610 at 821.506 kb on - strand, within BPHYT_RS03610 at 821.521 kb on - strand, within BPHYT_RS03610 at 821.545 kb on - strand, within BPHYT_RS03610 at 821.834 kb on - strand, within BPHYT_RS03610 at 822.089 kb on - strand at 822.089 kb on - strand at 822.631 kb on - strand at 822.631 kb on - strand at 822.631 kb on - strand at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.890 kb on + strand, within BPHYT_RS03620
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 820,417 - -0.7 820,417 - +0.5 820,417 - +0.6 820,514 - -2.7 821,484 - BPHYT_RS03610 0.18 +0.7 821,500 - BPHYT_RS03610 0.21 +0.1 821,506 - BPHYT_RS03610 0.21 +1.0 821,521 - BPHYT_RS03610 0.24 +1.3 821,545 - BPHYT_RS03610 0.27 -0.0 821,834 - BPHYT_RS03610 0.70 +0.9 822,089 - -0.0 822,089 - +0.8 822,631 - +0.1 822,631 - -0.2 822,631 - +1.4 822,818 - BPHYT_RS03620 0.12 +0.4 822,818 - BPHYT_RS03620 0.12 +0.6 822,818 - BPHYT_RS03620 0.12 +1.1 822,818 - BPHYT_RS03620 0.12 -0.1 822,890 + BPHYT_RS03620 0.16 +1.0
Or see this region's nucleotide sequence