Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01870 and BPHYT_RS01875 are separated by 284 nucleotides BPHYT_RS01875 and BPHYT_RS01880 are separated by 252 nucleotides
BPHYT_RS01870: BPHYT_RS01870 - 6-aminohexanoate-cyclic-dimer hydrolase, at 424,698 to 426,218
_RS01870
BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201
_RS01875
BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554
_RS01880
Position (kb) 426
427
428 Strain fitness (log2 ratio) -2
-1
0
1
2 at 425.694 kb on - strand, within BPHYT_RS01870 at 425.792 kb on + strand, within BPHYT_RS01870 at 425.792 kb on + strand, within BPHYT_RS01870 at 425.793 kb on - strand, within BPHYT_RS01870 at 425.794 kb on + strand, within BPHYT_RS01870 at 425.794 kb on + strand, within BPHYT_RS01870 at 425.795 kb on - strand, within BPHYT_RS01870 at 426.070 kb on + strand at 426.168 kb on + strand at 426.169 kb on - strand at 426.361 kb on + strand at 426.370 kb on + strand at 426.370 kb on + strand at 426.371 kb on - strand at 426.504 kb on + strand at 426.567 kb on + strand at 426.567 kb on + strand at 426.568 kb on - strand at 426.612 kb on + strand, within BPHYT_RS01875 at 426.613 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 427.070 kb on - strand, within BPHYT_RS01875 at 427.144 kb on - strand at 427.908 kb on + strand, within BPHYT_RS01880 at 427.909 kb on - strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.917 kb on + strand, within BPHYT_RS01880 at 427.918 kb on - strand, within BPHYT_RS01880
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 425,694 - BPHYT_RS01870 0.65 +0.5 425,792 + BPHYT_RS01870 0.72 +1.2 425,792 + BPHYT_RS01870 0.72 +0.2 425,793 - BPHYT_RS01870 0.72 +1.0 425,794 + BPHYT_RS01870 0.72 +0.8 425,794 + BPHYT_RS01870 0.72 -0.4 425,795 - BPHYT_RS01870 0.72 -0.3 426,070 + +1.6 426,168 + -0.1 426,169 - -0.8 426,361 + +1.2 426,370 + +0.3 426,370 + -0.9 426,371 - +0.1 426,504 + +0.7 426,567 + +1.2 426,567 + -1.0 426,568 - -0.4 426,612 + BPHYT_RS01875 0.16 -0.3 426,613 - BPHYT_RS01875 0.16 -0.6 426,772 - BPHYT_RS01875 0.38 +0.6 426,772 - BPHYT_RS01875 0.38 -0.2 426,813 + BPHYT_RS01875 0.44 -1.7 426,813 + BPHYT_RS01875 0.44 -0.3 426,975 + BPHYT_RS01875 0.68 -1.1 426,975 + BPHYT_RS01875 0.68 -0.9 427,070 - BPHYT_RS01875 0.81 +0.3 427,144 - -0.0 427,908 + BPHYT_RS01880 0.41 +0.5 427,909 - BPHYT_RS01880 0.41 -1.0 427,910 + BPHYT_RS01880 0.41 -0.3 427,910 + BPHYT_RS01880 0.41 +0.2 427,910 + BPHYT_RS01880 0.41 -0.4 427,910 + BPHYT_RS01880 0.41 -0.0 427,910 + BPHYT_RS01880 0.41 +0.2 427,914 + BPHYT_RS01880 0.42 +1.8 427,914 + BPHYT_RS01880 0.42 +0.0 427,914 + BPHYT_RS01880 0.42 +0.2 427,914 + BPHYT_RS01880 0.42 -0.4 427,914 + BPHYT_RS01880 0.42 +0.2 427,914 + BPHYT_RS01880 0.42 +0.4 427,914 + BPHYT_RS01880 0.42 +0.9 427,915 - BPHYT_RS01880 0.42 -1.7 427,915 - BPHYT_RS01880 0.42 -2.1 427,915 - BPHYT_RS01880 0.42 +0.3 427,917 + BPHYT_RS01880 0.42 -0.1 427,918 - BPHYT_RS01880 0.42 -0.7
Or see this region's nucleotide sequence