Experiment: LB_DAP agar plate, outgrowth in LB_gent
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01440 and BPHYT_RS01445 are separated by 237 nucleotides BPHYT_RS01445 and BPHYT_RS01450 are separated by 89 nucleotides BPHYT_RS01450 and BPHYT_RS01455 are separated by 244 nucleotides BPHYT_RS01455 and BPHYT_RS01460 are separated by 31 nucleotides
BPHYT_RS01440: BPHYT_RS01440 - DNA topoisomerase III, at 328,319 to 330,988
_RS01440
BPHYT_RS01445: BPHYT_RS01445 - thioredoxin, at 331,226 to 331,603
_RS01445
BPHYT_RS01450: BPHYT_RS01450 - DNA-binding protein, at 331,693 to 332,961
_RS01450
BPHYT_RS01455: BPHYT_RS01455 - peptide deformylase, at 333,206 to 333,709
_RS01455
BPHYT_RS01460: BPHYT_RS01460 - methionyl-tRNA formyltransferase, at 333,741 to 334,727
_RS01460
Position (kb) 331
332
333 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 331.008 kb on - strand at 331.008 kb on - strand at 331.008 kb on - strand at 331.008 kb on - strand at 331.008 kb on - strand at 331.008 kb on - strand at 331.090 kb on - strand at 331.090 kb on - strand at 331.092 kb on - strand at 331.092 kb on - strand at 331.092 kb on - strand at 331.126 kb on + strand at 331.142 kb on + strand at 331.143 kb on - strand at 331.143 kb on - strand at 331.648 kb on - strand at 331.648 kb on - strand at 331.648 kb on - strand at 331.705 kb on - strand at 331.705 kb on - strand at 331.778 kb on - strand at 331.778 kb on - strand at 331.918 kb on + strand, within BPHYT_RS01450 at 331.954 kb on + strand, within BPHYT_RS01450 at 331.955 kb on - strand, within BPHYT_RS01450 at 331.996 kb on - strand, within BPHYT_RS01450 at 332.400 kb on - strand, within BPHYT_RS01450 at 332.629 kb on + strand, within BPHYT_RS01450 at 332.629 kb on + strand, within BPHYT_RS01450 at 332.629 kb on + strand, within BPHYT_RS01450 at 332.629 kb on + strand, within BPHYT_RS01450 at 332.630 kb on - strand, within BPHYT_RS01450 at 332.630 kb on - strand, within BPHYT_RS01450 at 332.630 kb on - strand, within BPHYT_RS01450 at 332.630 kb on - strand, within BPHYT_RS01450 at 332.630 kb on - strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.631 kb on + strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.632 kb on - strand, within BPHYT_RS01450 at 332.633 kb on + strand, within BPHYT_RS01450 at 332.633 kb on + strand, within BPHYT_RS01450 at 332.634 kb on - strand, within BPHYT_RS01450 at 332.634 kb on - strand, within BPHYT_RS01450 at 332.634 kb on - strand, within BPHYT_RS01450 at 332.635 kb on + strand, within BPHYT_RS01450 at 332.635 kb on + strand, within BPHYT_RS01450 at 332.636 kb on - strand, within BPHYT_RS01450 at 332.807 kb on - strand, within BPHYT_RS01450 at 332.979 kb on - strand at 332.979 kb on - strand at 332.979 kb on - strand at 332.979 kb on - strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.111 kb on + strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.112 kb on - strand at 333.213 kb on + strand at 333.644 kb on + strand, within BPHYT_RS01455 at 333.707 kb on + strand at 333.707 kb on + strand at 333.707 kb on + strand at 333.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB_gent remove 331,008 - +0.7 331,008 - +0.7 331,008 - -0.4 331,008 - +0.5 331,008 - +0.0 331,008 - +0.4 331,090 - -0.2 331,090 - +1.2 331,092 - -0.0 331,092 - +0.0 331,092 - +0.2 331,126 + -2.0 331,142 + -0.6 331,143 - +0.4 331,143 - +1.1 331,648 - +0.5 331,648 - -0.0 331,648 - +2.2 331,705 - +0.7 331,705 - +0.6 331,778 - -0.1 331,778 - +0.3 331,918 + BPHYT_RS01450 0.18 -0.1 331,954 + BPHYT_RS01450 0.21 +0.2 331,955 - BPHYT_RS01450 0.21 -0.8 331,996 - BPHYT_RS01450 0.24 +0.3 332,400 - BPHYT_RS01450 0.56 +0.2 332,629 + BPHYT_RS01450 0.74 -0.2 332,629 + BPHYT_RS01450 0.74 -0.9 332,629 + BPHYT_RS01450 0.74 +0.6 332,629 + BPHYT_RS01450 0.74 +0.2 332,630 - BPHYT_RS01450 0.74 -0.6 332,630 - BPHYT_RS01450 0.74 +0.2 332,630 - BPHYT_RS01450 0.74 +0.4 332,630 - BPHYT_RS01450 0.74 +0.7 332,630 - BPHYT_RS01450 0.74 +0.6 332,631 + BPHYT_RS01450 0.74 -0.4 332,631 + BPHYT_RS01450 0.74 -0.1 332,631 + BPHYT_RS01450 0.74 +0.2 332,631 + BPHYT_RS01450 0.74 +0.3 332,631 + BPHYT_RS01450 0.74 -0.3 332,631 + BPHYT_RS01450 0.74 -0.8 332,631 + BPHYT_RS01450 0.74 -1.3 332,631 + BPHYT_RS01450 0.74 -1.2 332,631 + BPHYT_RS01450 0.74 -0.1 332,632 - BPHYT_RS01450 0.74 -0.6 332,632 - BPHYT_RS01450 0.74 +0.4 332,632 - BPHYT_RS01450 0.74 -0.2 332,632 - BPHYT_RS01450 0.74 -0.1 332,632 - BPHYT_RS01450 0.74 -0.3 332,632 - BPHYT_RS01450 0.74 +0.6 332,632 - BPHYT_RS01450 0.74 -0.1 332,632 - BPHYT_RS01450 0.74 +0.2 332,632 - BPHYT_RS01450 0.74 -0.4 332,632 - BPHYT_RS01450 0.74 +0.2 332,633 + BPHYT_RS01450 0.74 +0.5 332,633 + BPHYT_RS01450 0.74 -0.0 332,634 - BPHYT_RS01450 0.74 +0.2 332,634 - BPHYT_RS01450 0.74 +0.6 332,634 - BPHYT_RS01450 0.74 +0.5 332,635 + BPHYT_RS01450 0.74 -0.2 332,635 + BPHYT_RS01450 0.74 -1.0 332,636 - BPHYT_RS01450 0.74 +0.1 332,807 - BPHYT_RS01450 0.88 -0.4 332,979 - +1.2 332,979 - +0.1 332,979 - -1.8 332,979 - +0.6 333,111 + +0.5 333,111 + -0.7 333,111 + +0.3 333,111 + -1.1 333,112 - +0.6 333,112 - -1.3 333,112 - +0.3 333,112 - -0.7 333,112 - +0.9 333,112 - +0.2 333,112 - +0.6 333,112 - +0.1 333,112 - -0.2 333,213 + -2.4 333,644 + BPHYT_RS01455 0.87 -3.1 333,707 + -0.2 333,707 + +0.8 333,707 + +0.2 333,707 + -1.2
Or see this region's nucleotide sequence