Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35780

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35775 and BPHYT_RS35780 are separated by 26 nucleotidesBPHYT_RS35780 and BPHYT_RS35785 overlap by 4 nucleotidesBPHYT_RS35785 and BPHYT_RS35790 are separated by 148 nucleotides BPHYT_RS35775: BPHYT_RS35775 - ribulose bisphosphate carboxylase, at 3,615,746 to 3,617,044 _RS35775 BPHYT_RS35780: BPHYT_RS35780 - Hrp-dependent type III effector protein, at 3,617,071 to 3,618,417 _RS35780 BPHYT_RS35785: BPHYT_RS35785 - glucose-1-dehydrogenase, at 3,618,414 to 3,619,214 _RS35785 BPHYT_RS35790: BPHYT_RS35790 - hypothetical protein, at 3,619,363 to 3,619,518 _RS35790 Position (kb) 3617 3618 3619Strain fitness (log2 ratio) -2 -1 0 1at 3616.585 kb on - strand, within BPHYT_RS35775at 3616.793 kb on - strand, within BPHYT_RS35775at 3616.996 kb on + strandat 3617.367 kb on + strand, within BPHYT_RS35780at 3617.473 kb on + strand, within BPHYT_RS35780at 3617.604 kb on - strand, within BPHYT_RS35780at 3617.840 kb on - strand, within BPHYT_RS35780at 3617.963 kb on + strand, within BPHYT_RS35780at 3618.041 kb on - strand, within BPHYT_RS35780at 3618.440 kb on - strandat 3618.608 kb on + strand, within BPHYT_RS35785at 3618.609 kb on - strand, within BPHYT_RS35785at 3618.725 kb on - strand, within BPHYT_RS35785at 3618.920 kb on + strand, within BPHYT_RS35785at 3619.237 kb on + strandat 3619.238 kb on - strandat 3619.262 kb on + strandat 3619.262 kb on + strandat 3619.262 kb on + strandat 3619.263 kb on - strandat 3619.340 kb on + strandat 3619.340 kb on + strandat 3619.341 kb on - strandat 3619.341 kb on - strandat 3619.350 kb on + strandat 3619.365 kb on - strandat 3619.366 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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3,616,585 - BPHYT_RS35775 0.65 -0.4
3,616,793 - BPHYT_RS35775 0.81 -0.4
3,616,996 + +0.3
3,617,367 + BPHYT_RS35780 0.22 -0.4
3,617,473 + BPHYT_RS35780 0.30 -0.8
3,617,604 - BPHYT_RS35780 0.40 -0.2
3,617,840 - BPHYT_RS35780 0.57 -0.2
3,617,963 + BPHYT_RS35780 0.66 -0.1
3,618,041 - BPHYT_RS35780 0.72 -0.1
3,618,440 - -0.2
3,618,608 + BPHYT_RS35785 0.24 -0.3
3,618,609 - BPHYT_RS35785 0.24 -1.9
3,618,725 - BPHYT_RS35785 0.39 -1.3
3,618,920 + BPHYT_RS35785 0.63 -1.4
3,619,237 + +0.3
3,619,238 - +0.1
3,619,262 + -0.7
3,619,262 + -0.1
3,619,262 + +0.8
3,619,263 - -0.2
3,619,340 + -0.9
3,619,340 + -0.4
3,619,341 - -0.3
3,619,341 - +0.3
3,619,350 + +1.6
3,619,365 - -0.5
3,619,366 + -0.0

Or see this region's nucleotide sequence