Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13525

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13520 and BPHYT_RS13525 are separated by 17 nucleotidesBPHYT_RS13525 and BPHYT_RS13530 are separated by 94 nucleotides BPHYT_RS13520: BPHYT_RS13520 - isopropylmalate isomerase, at 3,084,454 to 3,085,869 _RS13520 BPHYT_RS13525: BPHYT_RS13525 - alpha/beta hydrolase, at 3,085,887 to 3,086,765 _RS13525 BPHYT_RS13530: BPHYT_RS13530 - LysR family transcriptional regulator, at 3,086,860 to 3,087,762 _RS13530 Position (kb) 3085 3086 3087Strain fitness (log2 ratio) -2 -1 0 1at 3084.889 kb on - strand, within BPHYT_RS13520at 3084.890 kb on + strand, within BPHYT_RS13520at 3085.034 kb on + strand, within BPHYT_RS13520at 3085.035 kb on - strand, within BPHYT_RS13520at 3085.097 kb on + strand, within BPHYT_RS13520at 3085.268 kb on + strand, within BPHYT_RS13520at 3085.464 kb on - strand, within BPHYT_RS13520at 3085.763 kb on + strandat 3085.764 kb on - strandat 3085.948 kb on + strandat 3085.948 kb on + strandat 3086.793 kb on + strandat 3086.794 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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3,084,889 - BPHYT_RS13520 0.31 +0.9
3,084,890 + BPHYT_RS13520 0.31 +0.1
3,085,034 + BPHYT_RS13520 0.41 -2.2
3,085,035 - BPHYT_RS13520 0.41 -1.2
3,085,097 + BPHYT_RS13520 0.45 -1.4
3,085,268 + BPHYT_RS13520 0.57 +0.4
3,085,464 - BPHYT_RS13520 0.71 -2.2
3,085,763 + -0.7
3,085,764 - -0.2
3,085,948 + +0.6
3,085,948 + +0.2
3,086,793 + -0.5
3,086,794 - +0.6

Or see this region's nucleotide sequence