Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10715

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10710 and BPHYT_RS10715 are separated by 236 nucleotidesBPHYT_RS10715 and BPHYT_RS10720 are separated by 67 nucleotidesBPHYT_RS10720 and BPHYT_RS10725 are separated by 18 nucleotidesBPHYT_RS10725 and BPHYT_RS10730 overlap by 4 nucleotides BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776 _RS10710 BPHYT_RS10715: BPHYT_RS10715 - acyl-CoA dehydrogenase, at 2,439,013 to 2,440,191 _RS10715 BPHYT_RS10720: BPHYT_RS10720 - hypothetical protein, at 2,440,259 to 2,440,636 _RS10720 BPHYT_RS10725: BPHYT_RS10725 - polyketide cyclase, at 2,440,655 to 2,441,170 _RS10725 BPHYT_RS10730: BPHYT_RS10730 - Glu-tRNA amidotransferase, at 2,441,167 to 2,442,291 _RS10730 Position (kb) 2439 2440 2441Strain fitness (log2 ratio) -2 -1 0 1at 2438.807 kb on + strandat 2438.927 kb on + strandat 2439.333 kb on + strand, within BPHYT_RS10715at 2439.698 kb on - strand, within BPHYT_RS10715at 2440.136 kb on - strandat 2440.136 kb on - strandat 2440.189 kb on + strandat 2440.217 kb on - strandat 2440.475 kb on + strand, within BPHYT_RS10720at 2440.554 kb on - strand, within BPHYT_RS10720at 2440.748 kb on - strand, within BPHYT_RS10725at 2441.018 kb on + strand, within BPHYT_RS10725

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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2,438,807 + +0.2
2,438,927 + +0.4
2,439,333 + BPHYT_RS10715 0.27 -0.0
2,439,698 - BPHYT_RS10715 0.58 -0.7
2,440,136 - +1.7
2,440,136 - -2.7
2,440,189 + +1.6
2,440,217 - +0.7
2,440,475 + BPHYT_RS10720 0.57 +1.0
2,440,554 - BPHYT_RS10720 0.78 -1.1
2,440,748 - BPHYT_RS10725 0.18 +0.5
2,441,018 + BPHYT_RS10725 0.70 +0.1

Or see this region's nucleotide sequence