Experiment: LB_DAP agar plate, outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02795 and BPHYT_RS02800 are separated by 228 nucleotides BPHYT_RS02800 and BPHYT_RS02805 are separated by 25 nucleotides BPHYT_RS02805 and BPHYT_RS02810 are separated by 98 nucleotides
BPHYT_RS02795: BPHYT_RS02795 - glycosyl transferase, at 627,272 to 628,243
_RS02795
BPHYT_RS02800: BPHYT_RS02800 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 628,472 to 629,170
_RS02800
BPHYT_RS02805: BPHYT_RS02805 - maleylacetoacetate isomerase, at 629,196 to 629,840
_RS02805
BPHYT_RS02810: BPHYT_RS02810 - cell division protein FtsY, at 629,939 to 631,099
_RS02810
Position (kb) 628
629
630 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 627.499 kb on + strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.952 kb on - strand, within BPHYT_RS02795 at 627.952 kb on - strand, within BPHYT_RS02795 at 628.129 kb on - strand, within BPHYT_RS02795 at 628.129 kb on - strand, within BPHYT_RS02795 at 628.294 kb on + strand at 628.295 kb on - strand at 628.321 kb on + strand at 628.322 kb on - strand at 628.330 kb on + strand at 628.330 kb on + strand at 628.331 kb on - strand at 628.416 kb on + strand at 628.459 kb on - strand at 628.459 kb on - strand at 628.479 kb on - strand at 628.479 kb on - strand at 628.479 kb on - strand at 628.482 kb on + strand at 628.482 kb on + strand at 628.483 kb on - strand at 628.483 kb on - strand at 628.483 kb on - strand at 628.553 kb on + strand, within BPHYT_RS02800 at 628.553 kb on + strand, within BPHYT_RS02800 at 628.554 kb on - strand, within BPHYT_RS02800 at 628.571 kb on + strand, within BPHYT_RS02800 at 628.790 kb on + strand, within BPHYT_RS02800 at 628.790 kb on + strand, within BPHYT_RS02800 at 628.790 kb on + strand, within BPHYT_RS02800 at 628.791 kb on - strand, within BPHYT_RS02800 at 629.030 kb on + strand, within BPHYT_RS02800 at 629.031 kb on - strand, within BPHYT_RS02800 at 629.031 kb on - strand, within BPHYT_RS02800 at 629.031 kb on - strand, within BPHYT_RS02800 at 629.205 kb on + strand at 629.205 kb on + strand at 629.205 kb on + strand at 629.205 kb on + strand at 629.206 kb on - strand at 629.206 kb on - strand at 629.206 kb on - strand at 629.206 kb on - strand at 629.206 kb on - strand at 629.211 kb on + strand at 629.212 kb on - strand at 629.232 kb on + strand at 629.271 kb on + strand, within BPHYT_RS02805 at 629.271 kb on + strand, within BPHYT_RS02805 at 629.277 kb on + strand, within BPHYT_RS02805 at 629.278 kb on - strand, within BPHYT_RS02805 at 629.472 kb on + strand, within BPHYT_RS02805 at 629.542 kb on - strand, within BPHYT_RS02805 at 629.571 kb on + strand, within BPHYT_RS02805 at 629.571 kb on + strand, within BPHYT_RS02805 at 629.572 kb on - strand, within BPHYT_RS02805 at 629.741 kb on + strand, within BPHYT_RS02805 at 629.742 kb on - strand, within BPHYT_RS02805 at 629.747 kb on + strand, within BPHYT_RS02805 at 629.747 kb on + strand, within BPHYT_RS02805 at 629.748 kb on - strand, within BPHYT_RS02805 at 629.772 kb on + strand at 629.772 kb on + strand, within BPHYT_RS02805 at 629.773 kb on - strand, within BPHYT_RS02805 at 629.838 kb on + strand at 629.839 kb on - strand at 629.904 kb on + strand at 629.905 kb on - strand at 629.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB remove 627,499 + BPHYT_RS02795 0.23 -0.6 627,611 - BPHYT_RS02795 0.35 -0.1 627,611 - BPHYT_RS02795 0.35 +0.3 627,612 + BPHYT_RS02795 0.35 -0.0 627,612 + BPHYT_RS02795 0.35 -0.3 627,612 + BPHYT_RS02795 0.35 -1.4 627,613 - BPHYT_RS02795 0.35 -0.3 627,613 - BPHYT_RS02795 0.35 +0.9 627,613 - BPHYT_RS02795 0.35 +0.1 627,952 - BPHYT_RS02795 0.70 -0.5 627,952 - BPHYT_RS02795 0.70 -0.0 628,129 - BPHYT_RS02795 0.88 +3.1 628,129 - BPHYT_RS02795 0.88 +0.2 628,294 + -0.2 628,295 - +0.6 628,321 + -0.9 628,322 - -0.0 628,330 + +0.3 628,330 + +1.8 628,331 - +0.1 628,416 + -2.9 628,459 - +0.5 628,459 - -0.4 628,479 - -0.5 628,479 - +0.3 628,479 - -0.7 628,482 + -0.2 628,482 + -0.1 628,483 - +0.2 628,483 - -0.3 628,483 - -0.4 628,553 + BPHYT_RS02800 0.12 +1.3 628,553 + BPHYT_RS02800 0.12 +0.3 628,554 - BPHYT_RS02800 0.12 +1.3 628,571 + BPHYT_RS02800 0.14 -0.0 628,790 + BPHYT_RS02800 0.45 +1.1 628,790 + BPHYT_RS02800 0.45 +0.0 628,790 + BPHYT_RS02800 0.45 +1.0 628,791 - BPHYT_RS02800 0.46 +0.1 629,030 + BPHYT_RS02800 0.80 +0.7 629,031 - BPHYT_RS02800 0.80 +1.3 629,031 - BPHYT_RS02800 0.80 +0.8 629,031 - BPHYT_RS02800 0.80 -0.6 629,205 + +0.2 629,205 + +1.3 629,205 + -1.3 629,205 + +0.4 629,206 - -1.1 629,206 - +0.3 629,206 - +0.4 629,206 - -0.9 629,206 - -0.2 629,211 + -0.5 629,212 - -0.0 629,232 + +0.3 629,271 + BPHYT_RS02805 0.12 -2.3 629,271 + BPHYT_RS02805 0.12 +1.7 629,277 + BPHYT_RS02805 0.13 +0.2 629,278 - BPHYT_RS02805 0.13 +0.5 629,472 + BPHYT_RS02805 0.43 +0.6 629,542 - BPHYT_RS02805 0.54 +1.2 629,571 + BPHYT_RS02805 0.58 +0.1 629,571 + BPHYT_RS02805 0.58 +0.1 629,572 - BPHYT_RS02805 0.58 -0.1 629,741 + BPHYT_RS02805 0.84 -2.1 629,742 - BPHYT_RS02805 0.85 +0.1 629,747 + BPHYT_RS02805 0.85 -0.7 629,747 + BPHYT_RS02805 0.85 -0.7 629,748 - BPHYT_RS02805 0.86 +0.1 629,772 + +0.7 629,772 + BPHYT_RS02805 0.89 -0.7 629,773 - BPHYT_RS02805 0.89 -0.0 629,838 + -0.2 629,839 - -0.2 629,904 + -0.1 629,905 - +0.3 629,917 + -0.0
Or see this region's nucleotide sequence