Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00050

Experiment: LB_DAP agar plate, outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS00040 and BPHYT_RS00045 overlap by 4 nucleotidesBPHYT_RS00045 and BPHYT_RS00050 are separated by 18 nucleotidesBPHYT_RS00050 and BPHYT_RS00055 are separated by 72 nucleotidesBPHYT_RS00055 and BPHYT_RS00060 are separated by 104 nucleotides BPHYT_RS00040: BPHYT_RS00040 - hypothetical protein, at 11,305 to 14,187 _RS00040 BPHYT_RS00045: BPHYT_RS00045 - hypothetical protein, at 14,184 to 14,939 _RS00045 BPHYT_RS00050: BPHYT_RS00050 - hypothetical protein, at 14,958 to 15,692 _RS00050 BPHYT_RS00055: BPHYT_RS00055 - hypothetical protein, at 15,765 to 16,016 _RS00055 BPHYT_RS00060: BPHYT_RS00060 - hypothetical protein, at 16,121 to 18,958 _RS00060 Position (kb) 14 15 16Strain fitness (log2 ratio) -2 -1 0 1at 13.971 kb on + strandat 13.971 kb on + strandat 13.971 kb on + strandat 13.972 kb on - strandat 14.937 kb on + strandat 15.244 kb on + strand, within BPHYT_RS00050at 15.289 kb on - strand, within BPHYT_RS00050at 15.327 kb on + strand, within BPHYT_RS00050at 15.585 kb on + strand, within BPHYT_RS00050at 15.585 kb on + strand, within BPHYT_RS00050at 15.585 kb on + strand, within BPHYT_RS00050at 15.585 kb on + strand, within BPHYT_RS00050at 15.664 kb on - strandat 15.673 kb on - strandat 15.723 kb on + strandat 15.723 kb on + strandat 15.724 kb on - strandat 15.794 kb on + strand, within BPHYT_RS00055at 15.795 kb on - strand, within BPHYT_RS00055at 16.052 kb on - strandat 16.187 kb on + strandat 16.277 kb on + strandat 16.278 kb on - strandat 16.278 kb on - strandat 16.310 kb on + strandat 16.311 kb on - strandat 16.311 kb on - strandat 16.319 kb on + strandat 16.346 kb on + strandat 16.346 kb on + strandat 16.359 kb on - strandat 16.469 kb on - strand, within BPHYT_RS00060at 16.524 kb on - strand, within BPHYT_RS00060at 16.553 kb on + strand, within BPHYT_RS00060at 16.553 kb on + strand, within BPHYT_RS00060at 16.679 kb on + strand, within BPHYT_RS00060at 16.680 kb on - strand, within BPHYT_RS00060at 16.680 kb on - strand, within BPHYT_RS00060

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_DAP agar plate, outgrowth in LB
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13,971 + +0.1
13,971 + -0.7
13,971 + -0.2
13,972 - +0.8
14,937 + +0.1
15,244 + BPHYT_RS00050 0.39 -0.1
15,289 - BPHYT_RS00050 0.45 -0.1
15,327 + BPHYT_RS00050 0.50 +0.0
15,585 + BPHYT_RS00050 0.85 +0.2
15,585 + BPHYT_RS00050 0.85 +0.2
15,585 + BPHYT_RS00050 0.85 -0.4
15,585 + BPHYT_RS00050 0.85 +0.8
15,664 - -0.8
15,673 - -0.0
15,723 + +0.9
15,723 + +1.3
15,724 - +0.4
15,794 + BPHYT_RS00055 0.12 -2.7
15,795 - BPHYT_RS00055 0.12 -0.9
16,052 - +1.2
16,187 + -1.6
16,277 + -0.5
16,278 - -0.4
16,278 - +0.1
16,310 + -0.3
16,311 - +0.6
16,311 - -0.3
16,319 + -0.0
16,346 + -0.1
16,346 + -1.4
16,359 - -0.6
16,469 - BPHYT_RS00060 0.12 +0.2
16,524 - BPHYT_RS00060 0.14 -0.7
16,553 + BPHYT_RS00060 0.15 -0.2
16,553 + BPHYT_RS00060 0.15 +0.9
16,679 + BPHYT_RS00060 0.20 -0.1
16,680 - BPHYT_RS00060 0.20 +0.2
16,680 - BPHYT_RS00060 0.20 +0.5

Or see this region's nucleotide sequence